Hampered binding to blood serum albumin and antimicrobial activity of ibuprofen immobilised on magnetic nanoparticles Fe3O4

摘要

Drug distribution in an organism depends largely on the binding degree of drugs with blood serum proteins like albumin. Only free fraction of the drug can induce a therapeutic effect. A different affinity of particular drugs to proteins is also one of the reasons of the occurrence of drug interactions. It was decided to investigate if the immobilisation of some model drugs on magnetic nanoparticles Fe3O4 (MNPs)' would influence their activity and protect from binding to albumin. It was proposed to attach the drugs such as ibuprofen (IB), acetylsalicylic acid and naproxen to the MNPs coated with (3-aminopropyl)triethoxysilane' by electrostatic interaction since carboxyl groups of the drugs can form ionic bonds with amino groups of the silane. The characterisation of morphology of the obtained nanostructures was also carried out. The drug release studies showed very slow diffusion of the drugs from the MNPs surface. It has been demonstrated that IB immobilised on MNPs exhibits high antimicrobial activity even in its low concentration and in the presence of albumin - in contrast to the unattached form of the drug.