A new ELISA method for the measurement of total alpha(2)-plasmin inhibitor level in human body fluids

摘要

The ever-increasing research efforts to develop new antithrombotic therapies have led to the reassessment of the role of alpha-2-plasmin inhibitor (alpha(2)-PI) in pathological conditions. In particular, experimental stroke studies have suggested correlation between increased free alpha 2-PI level and mortality. However there are only a small number of well-characterized and specific assays available for the measurements of free alpha(2)-PI. In plasma alpha(2)-PI undergoes both N- and/or C-terminal cleavages resulting four isoforms with modified susceptibility to FXIII catalyzed cross-linking to fibrin and/or loss of plasmin(ogen) binding.