Determination of Ochratoxin A in Turkish Wines

摘要

A validated, simple and highly sensitive analytical method was described for the determination of Ochratoxin A (OTA) in various wines produced in Turkey. OTA concentrations of 25 wine samples were examined using high performance liquid chroma-tography coupled with fluorescence detection. OTA and diflunisal (internal standard) were separated using isocratic elution mode with a reversed phase Nucleosil~RC_(18) column. The mobile phase consisted of CH_3CN : H_2O : CH_3COOH (50 : 48 : 2, v/v/v) was pumped at a flow rate of 0.4 mL/min. The analytes were detected at 330 nm excitation and 450 nm emission wavelengths within an average time of 13 min. Samples were prepared by simply filtrating the wine samples through a 0.2 urn filter and injected into the system without further extraction or concentration steps. OTA was detected inmug/L levels with adequate chromatographic resolution. It was found that the amount of OTA was higher than the permitted limits (< 2 mug/L) in 14 out of 25 samples, especially in red wines.