Effects of genotype and 2,4,5-T concentrations on callus induction, shoot formation and plant regeneration from young inflorescences in orchardgrass (Dactylis glomerata L.)

摘要

This study was carried out to determine the effects of MS medium supplemented with 2, 4, 6 and 8 mg 11 of 2,4,5-T (2,4,5-trichlorophenoxyacetic acid) on young inflorescences of five ecotypes of orchardgrass (Dactylis glomerata L.). The results showedthat there was a significant interaction between ecotypes and 2,4,5-T concentrations in all characteristics studied. Depending on the ecotypes, rate of callus induction (%), rate of shoot formation (%). callus weight (mg/per Petri dish) and number of regenerates per inflorescence segment varied and ranged 6.3-37.5%, 7.8-51.6%, 13.1-115.4 mg and 0.3-1.1, respectively. Rates of callus induction and shoot formation as well as callus weight were also significantly influenced by the 2,4,5-T concentrations. The explants cultured on MS medium containing 4 mg~(-1) of 2,4,5-T showed highest callus induction rate of 53.8 % with callus weight of 170.4 mg/Petri dish and 1.6 shoots per explant. It was concluded that best regeneration from young inflorescence explant of orchardgrass (D. glomerata) could be obtained on MS medium containing 4 mg 1of 2,4,5-T and the developed protocol will help in easy multiplication of orchard grass in future breeding studies.