Purification and characterization of fucose-containing sulphated polysaccharides from Sargassum tenerrimum and their biological activity

摘要

The crude sulphated polysaccharides (ST-D) were extracted from Sargassum tenerrimum and purified using DEAE-Sepharose anion-exchange chromatography and yielded three fractions (ST-DFI 46.2% (w/w), ST-DFII 51.6% (w/w) and ST-DFIII 2.2% (w/w)). The monosaccharide composition analysis showed fucose (40-43%) as the major sugar. The apparent molecular weight of ST-DF1, ST-DFII and ST-DFIII was found to be 233, 286 and 88 kDa, respectively. Structural investigation analysis by FTIR and NMR revealed the presence of sulphated polysaccharides with (1,3)-linked fucose with sulphation at C4 and C2 position. The ST-DFIII was the most biologically active fraction. It had the highest anticoagulant (APTT 265 +/- 2.7 s) and radical scavenging activity (superoxide radical IC50 26.1 +/- 0.15 mu g mL(-1)). ST-DFII was slightly less active than ST-DFIII but could be obtained in higher quantity. These results suggest that ST-DFII from S. tenerrimum could become a promising entity as nutraceutical molecule.