Host range of cotton leaf curl virus disease

摘要

Cotton is the major commercial cash crop which greatly affects the Indian economy. Cotton leaf curl disease (CLCuD) has become a prominent and potential threat for cotton production due to its regular appearance in cotton growing belt. Availability ofseveral alternate hosts is the main reason behind the consistent presence of its causal agent Cotton leaf curl virus (CLCuD). An experiment was performed at Cotton Section, CCS HAU, Hisar to determine the host range of CLCuD. Samples of weeds/vegetableswere collected on the basis of visual CLCuD symptoms before sowing, during growing and after picking of cotton crop. Genomic DNA of the leaf samples were used for PCR amplification using a set of primers for the conserved sequence of coat protein regionof several cotton leaf curl isolates. Among several samples genomic DNA of Solenum melongina, Parthenium spp., Euphorbia spp., Solarium lycopercisum, Convolvulus arvensis, Aeruca sativa,Croton sprucifera and Lantana camara during and before sowing of cotton, Abutilone indicum, Achyrenthes aspera and Croton sprucefera during cotton growing season and Canabis sativa, Croton sprucifera and Coronopus didymus after picking of cotton showed a positive amplified product of about 770bp. These are the host where the CLCuD present and survive before sowing of cotton, during cotton growing season and after picking of cotton crop.