Molecular chracterisation of cotton genotypes using SSR, ISSR and RAPD markers in relation to fiber quality traits

摘要

In the present study, twenty five cotton (Gossypium sp.) genotypes comprising of six G. hirsutum, three G. arboreum, one G. anomalam and one G. barbadense genotype, three intraspecific hybrids (G. hirsutum x G. hirsutum), two intraspecific hybrids (G.hirsutum x G. barbadense) and nine F6 lines of trispecific cross (G. arboreum GMSxG. anomalam xG. barbadense) were genotyped. Eighteen SSR markers reported to be linked to fiber quality QTLs, seven ISSR and six RAPD markers were used to assess genetic diversity and identification of markers associated with fiber quality traits. On pooled data analysis all genotypes were distinguished as separate clusters with similarity index range of 0.58-0.97 into eight broad groups. Highest divergence was observed in G. anomalum; three G. arboreum genotypes, introgression line 1-197, G. barbadense RHCb-001 and G. hirsutum Phule688. In both dendrogram and 2-D scatter plot, G. hirsutum genotypes grouped into two groups as per their contrasting fiber qualities. Low level of divergence was observed in genotypes with medium fiber quality traits that were grouped along with G. hirsutum x G. barbadense interspecies hybrids having extra-long fiber, good strength and fineness. Three, seven and twelve SSR markers were foundto be highly correlated with fibre length, strength and fineness respectively.SSR markers exhibiting highly significant correlation for fibre quality traits will assist breeders in transferring and maintaining valuable fibre quality traits during cultivar development.