Comparison of commercial methods of immunoblot, ELISA ELISA , and chemiluminescent immunoassay for detecting type‐specific herpes simplex viruses‐1 and ‐2 IgG

摘要

Background Serology for type‐specific herpes simplex virus ( HSV ) is based on the use of the respective glycoprotein G ( gG ). Methods Chemiluminescent immunoassay ( CLIA ; BIO ‐ FLASH ? , Biokit, Spain), ELISA (HerpeSelect ? , Focus, USA ), and immunoblot ( IB ; Virotech, Germany) for detecting HSV ‐1‐ and HSV ‐2‐specific IgG were compared using 384 serum samples received for HSV serology. The samples were classified as positive or negative according to a consensus criterion. Results For HSV ‐1, 262 samples were positive and 118 were negative (four samples were unclassifiable). IB showed agreement, sensitivity, and specificity values of 98.68%, 98.47% and 99.15%, respectively. The corresponding figures for CLIA and ELISA were 98.95%, 99.24% and 98.31%, and 98.16%, 99.62% and 94.92%, respectively. For HSV ‐2, 106 samples were positive and 278 were negative. Agreement, sensitivity, and specificity of IB were 99.48%, 98.11%, and 100%, respectively. The corresponding figures for CLIA and ELISA were 99.48%, 99.06% and 99.64%, and 98.18%, 99.06% and 97.84%, respectively. Conclusion The three methods showed excellent and equivalent performance characteristics for the detection of type‐specific IgG to HSV ‐1 and HSV ‐2.