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TABLET 'ELISA HSV-2-of IgG' TO DETERMINE immunoglobulin G to herpes simplex virus II TYPE

机译:平板电脑“ IgG的ELISA HSV-2-of”测定免疫球蛋白G对单纯疱疹病毒II型

摘要

The invention relates to the medical industry, particularly to biotechnology, Virology Immunology, for qualitative and semi-quantitative determination of immunoglobulins IgG class of herpes simplex virus type II. The technical result of the utility model is to overcome the drawbacks of analog and create a convenient and practical tablet to identify immunoglobulin G to herpes simplex virus II type quickly with a high probability (99% or more) in a rural or mobile laboratories, improved cooking test system, while increasing sensitivity and specificity. This result is achieved in that the plate for the determination of immunoglobulins IgG class to herpes simplex virus type 2, made of polystyrene with sorbed on the surface of the wells with antigen herpes virus type 2, and reagents, wherein the plate is configured as a housing and a set of removable strips with cylindrical or, made in the form of a parallelepiped hole, a height of 10 mm and a wall thickness of the well not less than 1 mm, as the antigen adsorbed on the surface of the wells, it is to contain a highly herpes virus type 2 proteins in de complete lysate purified antigen and an aqueous sodium salt solution: sodium phosphate dibasic 12-water, sodium phosphate monobasic 2-aqueous, sodium chloride, a detergent as Tween 20 or Tween 80 or sorbital A-20, with the addition of casein sodium azide , fetal serum, while it is packaged in a sealed package.
机译:本发明涉及医学工业,尤其涉及用于定性和半定量测定II型单纯疱疹病毒的免疫球蛋白IgG类的生物技术,病毒学免疫学。本实用新型的技术成果是克服了类似物的弊端,创造了一种方便实用的片剂,可以在农村或流动实验室中以很高的概率(99%以上)快速鉴定出针对单纯疱疹病毒II型的免疫球蛋白G,并得到了改进。烹饪测试系统,同时提高了灵敏度和特异性。获得该结果的原因在于,用于测定类型2的单纯疱疹病毒的免疫球蛋白IgG的平板(由聚苯乙烯吸附在孔表面的2型抗原的疱疹病毒制成)和试剂制成,其中该平板配置为外壳和一组带有圆柱形或以平行六面体孔形式制成的可移动条带,高度为10 mm,孔的壁厚不少于1 mm,因为抗原吸附在孔的表面,在完全裂解液纯化的抗原中含有高度疱疹2型病毒蛋白,以及钠盐水溶液:磷酸氢二钠12-水,磷酸氢二钠2-水溶液,氯化钠,去污剂(吐温20或吐温80或山梨醇) A-20,加酪蛋白叠氮化钠,胎儿血清,同时包装在密封包装中。

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