Captopril apparently increase and cisplatin apparently decrease human albumin concentration in artificial urinary solutions

摘要

Background Measurement of urinary albumin (HSA) is very important in diagnostic of kidney diseases. Much less is known about the possible impact of substances present in urine together with albumin on the results of measurements. Methods We investigated the effect of the presence of captopril and cisplatin in the solution on the result of the determination of HSA by native polyacrylamide gel electrophoresis. Protein conformation in the absence and presence of the drugs was examined using Fourier Transform Infrared Spectroscopy (FTIR). Results The presence of captopril apparently increases HSA concentration while cisplatin causes an apparent decrease in the HSA concentration. The presence of both drugs also influence the secondary structure forms of HSA albumin investigated by FTIR. Conclusion Both drugs tested in the concentration of human use can have an impact on the results of determination of albumin in urine which can influence clinical decision.