Optimization of on‐site cellulase preparation for efficient hydrolysis of atmospheric glycerol organosolv pretreated wheat straw

Sun Fubao; Mukasekuru Marie Rose; Tan Ling; Ren Junli; Huang Zhenxing; Ren Hongyan; Zhang Zhanying

摘要

Abstract <section xml:id="jctb5605-sec-0001" numbered="no"> <title type="main">BACKGROUND >The cost of using cellulase to effectively release fermentable sugars from lignocellulosic substrates is still a major impediment to development of the enzyme‐based ‘biorefinery’ industry. This study attempted to optimize a cellulase preparation from on‐site fermentation to efficiently hydrolyse atmospheric glycerol organosolv (AGO)‐pretreated wheat straw. </section> <section xml:id="jctb5605-sec-0002" numbered="no"> <title type="main">RESULTS > Addition of Cu 2+ , PEG10000, L‐ascorbic acid and trehalose in the fermentation medium significantly improved the cellulase production. Use of all the additives enabled enhancement to 45.5% of filter paper cellulase (FPA), 14.7% of β‐glucosidase (BG) and 32.4% of carboxymethyl cellulase (CMCase). With the on‐site production of crude cellulase, accessory enzymes (β‐glucosidase (BG), xylanase and lysozyme) and additives (tea saponin (TS) and cationic polyacrylamide (c‐PAM)) were found to facilitate the hydrolysis of AGO‐pretreated substrates effectively. The optimized cellulase mixture allowed 96% of enzymatic hydrolysis at 5 FPU g ‐1 for 48 h on addition of 11.5 U g ‐1 BG, 180 U g ‐1 xylanase, 45 U g ‐1 lysozyme, 10 mg g ‐1 TS and 2 mg g ‐1 C‐PAM, which was twice that of the crude cellulase broth. </section> <section xml:id="jctb5605-sec-0003" numbered="no"> <title type="main">CONCLUSION >Cellulase customization for specific subst </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> <div class="translation abstracttxt"> <span class="zhankaihshouqi fivelineshidden" id="abstract"> <span>机译：</span><Abstract XMLNS =“http://www.wiley.com/namespaces/wiley”type =“main”xml：lang =“en”> <title type =“main”>抽象</ title> <section XML：ID =“JCTB5605-SEC-0001”编号=“否”> <标题类型=“main”>背景</标题> >使用纤维素酶以有效释放来自木质纤维素基材的可发酵糖的成本仍然是发展的主要障碍基于酶的“生物质艺”产业。该研究试图优化从现场发酵的纤维素酶制剂，以有效地水解大气甘油有机溶胶（前一颗粒） - 普通的小麦秸秆。</ p> </ section> <章节XML：ID =“JCTB5605-SEC-0002”编号= “否”> <标题类型=“main”>结果</标题> >发酵培养基中的Cu 2 + </ sup>，PEG10000，L-抗坏血酸和海藻糖显着改善了纤维素酶生产。所有添加剂的使用使得增强至45.5％的滤纸纤维素酶（FPA），14.7％的β-葡糖苷酶（BG）和32.4％的羧甲基纤维素酶（CMCase）。发现粗纤维素酶的现场生产，辅助酶（β-葡糖苷酶（BG），木聚糖酶和溶菌酶）和添加剂（茶皂苷（TS）和阳离子聚丙烯酰胺（C-PAM））促进以往的水解 - 有效预处理的基材。优化的纤维素酶混合物在5fpu g -1 / sop>下允许96％的酶水解，加入11.5u g -1℃，180u g - 1 </ sup>木聚糖酶，45u g -1℃，10mg g -1和2mg g -1 -1 / sup> c-pam ，这是粗纤维素酶肉汤的两倍。 </ p> </ section> <section XML：ID =“JCTB5605-SEC-0003”编号=“否”> <标题类型=“main”>结论</标题> >特定字母的纤维素酶定制 </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> </div> <div class="record"> <h2 class="all_title" id="enpatent33" >著录项</h2> <ul> <li> <span class="lefttit">来源</span> <div style="width: 86%;vertical-align: text-top;display: inline-block;"> <a href='/journal-foreign-33908/'>《Journal of Chemical Technology & Biotechnology》</a> <b style="margin: 0 2px;">|</b><span>2018年第8期</span><b style="margin: 0 2px;">|</b><span>共10页</span> </div> </li> <li> <div class="author"> <span class="lefttit">作者</span> <p id="fAuthorthree" class="threelineshidden zhankaihshouqi"> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Sun Fubao&option=202" target="_blank" rel="nofollow">Sun Fubao;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Mukasekuru Marie Rose&option=202" target="_blank" rel="nofollow">Mukasekuru Marie Rose;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Tan Ling&option=202" target="_blank" rel="nofollow">Tan Ling;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Ren Junli&option=202" target="_blank" rel="nofollow">Ren Junli;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Huang Zhenxing&option=202" target="_blank" rel="nofollow">Huang Zhenxing;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Ren Hongyan&option=202" target="_blank" rel="nofollow">Ren Hongyan;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Zhang Zhanying&option=202" target="_blank" rel="nofollow">Zhang Zhanying;</a> </p> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zkzz" style="display: none;">展开▼</span> </div> </li> <li> <div style="display: flex;"> <span class="lefttit">作者单位</span> <div style="position: relative;margin-left: 3px;max-width: 639px;"> <div class="threelineshidden zhankaihshouqi" id="fOrgthree"> <p>Key Laboratory of Carbohydrate Chemistry and Biotechnology Ministry of EducationSchool of Biotechnology Jiangnan UniversityWuxi China;</p> <p>Key Laboratory of Carbohydrate Chemistry and Biotechnology Ministry of EducationSchool of Biotechnology Jiangnan UniversityWuxi China;</p> <p>Key Laboratory of Carbohydrate Chemistry and Biotechnology Ministry of EducationSchool of Biotechnology Jiangnan UniversityWuxi China;</p> <p>State Key Laboratory of Pulp and Paper EngineeringSouth China University of TechnologyGuangzhou China;</p> <p>Jiangsu Key Laboratory of Anaerobic Biotechnology School of Environmental and Civil EngineeringJiangnan UniversityWuxi China;</p> <p>Jiangsu Key Laboratory of Anaerobic Biotechnology School of Environmental and Civil EngineeringJiangnan UniversityWuxi China;</p> <p>Centre for Tropical Crops and BiocommoditiesQueensland University of Technology 2 George StBrisbane QLD Australia;</p> </div> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zhdw" style="display: none;">展开▼</span> </div> </div> </li> <li > <span class="lefttit">收录信息</span> <span style="width: 86%;vertical-align: text-top;display: inline-block;"></span> </li> <li> <span class="lefttit">原文格式</span> <span>PDF</span> </li> <li> <span class="lefttit">正文语种</span> <span>eng</span> </li> <li> <span class="lefttit">中图分类</span> <span><a href="https://www.zhangqiaokeyan.com/clc/180.html" title="生物工程学（生物技术）">生物工程学（生物技术）;</a><a href="https://www.zhangqiaokeyan.com/clc/20827.html" title="生物化学过程">生物化学过程;</a></span> </li> <li class="antistop"> <span class="lefttit">关键词</span> <p style="width: 86%;vertical-align: text-top;"> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=on‐site cellulase fermentation&option=203" rel="nofollow">on‐site cellulase fermentation;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Trichoderma reesei Rut‐C30&option=203" rel="nofollow">Trichoderma reesei Rut‐C30;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=accessory enzyme and additives&option=203" rel="nofollow">accessory enzyme and additives;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=atmospheric glycerol organosolv pretreatment&option=203" rel="nofollow">atmospheric glycerol organosolv pretreatment;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=wheat straw&option=203" rel="nofollow">wheat straw;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=enzymatic hydrolysis&option=203" rel="nofollow">enzymatic hydrolysis;</a> </p> <div class="translation"> 机译：现场纤维素酶发酵;Trichoderma Reesei Rut-C30;辅助酶和添加剂;大气甘油有机溶胶溶剂预处理;小麦秸秆;酶水解; </div> </li> </ul> </div> </div> <div class="literature cardcommon"> <div class="similarity "> <h3 class="all_title" id="enpatent66">相似文献</h3> <div class="similaritytab clearfix"> <ul> <li class="active" >外文文献</li> <li >中文文献</li> <li >专利</li> </ul> </div> <div class="similarity_details"> <ul > <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/journal-foreign-detail/0704025422938.html">Optimization of on‐site cellulase preparation for efficient hydrolysis of atmospheric glycerol organosolv pretreated wheat straw</a> <b>[J]</b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Sun Fubao&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Sun Fubao,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Mukasekuru Marie Rose&option=202" target="_blank" rel="nofollow" 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B. ,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Woidemann, A. &option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Woidemann, A. ,</a> <span>1998</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：通过商业真菌酶制剂的混合物水解来自湿氧化小麦秸秆的溶解的半纤维素</span> </p> </li> </ul> <ul style="display: none;"> <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/academic-journal-cn_hubei-agricultural-sciences_thesis/0201234094289.html">NaOH预处理小麦秸秆对纤维素酶水解的影响</a> <b>[J]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=马海元&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 马海元</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=林燕&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,林燕</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=张伟&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,张伟</a> <span> <a href="/journal-cn-11531/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 湖北农业科学 </a> </span> <span> . 2013</span><span>,第018期</span> </span> </div> </li> <li> <div> <b>2. </b><a class="enjiyixqcontent" href="/academic-journal-cn_transactions-chinese-society-agricultural-machinery_thesis/0201258187691.html">丙酸预处理小麦秸秆的纤维素水解动力学</a> <b>[J]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=田龙&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 田龙</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=马晓建&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,马晓建</a> <span> <a href="/journal-cn-6828/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 农业机械学报 </a> </span> <span> . 2012</span><span>,第009期</span> </span> </div> </li> <li> <div> <b>3. </b><a class="enjiyixqcontent" href="/academic-journal-cn_biomass-chemical-engineering_thesis/0201248147904.html">响应曲面法优化小麦秸秆纤维素酶水解条件</a> <b>[J]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=张伟&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 张伟</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=张琦&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,张琦</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=阮馨怡&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,阮馨怡</a> <span> <a href="/journal-cn-9196/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 生物质化学工程 </a> </span> <span> . 2015</span><span>,第002期</span> </span> </div> </li> <li> <div> <b>4. </b><a class="enjiyixqcontent" href="/academic-journal-cn_hubei-agricultural-sciences_thesis/0201281419626.html">过氧化氢和氢氧化钙联合预处理对小麦秸秆纤维素、半纤维素、木质素含量的影响</a> <b>[J]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=王泽家&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 王泽家</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=陈炀&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,陈炀</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=董媛媛&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,董媛媛</a> <span> <a href="/journal-cn-11531/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 湖北农业科学 </a> </span> <span> . 2020</span><span>,第022期</span> </span> </div> </li> <li> <div> <b>5. </b><a class="enjiyixqcontent" href="/academic-journal-cn_detail_thesis/0201295994761.html">优化预处理及脱毒工艺以制备玉米芯半纤维素水解液</a> <b>[J]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=王家旺&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 王家旺</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=杨晓峰&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,杨晓峰</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=刘国明&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,刘国明</a> <span> <a href="/journal-cn-56720/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 中国农学通报 </a> </span> <span> . 2020</span><span>,第20期</span> </span> </div> </li> <li> <div> <b>6. </b><a class="enjiyixqcontent" href="/academic-conference-cn_meeting-39758_thesis/020222402545.html">经济最优化的纤维素水解预处理手段的比较与思考</a> <b>[C]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Jian Liu&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 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href="/patent-detail/06120105518062.html">一种经济高效转化农秸秆纤维素糖的工业甘油常压预处理方法</a> <b>[P]</b> . <span> 中国专利： CN101323871A </span> <span> . 2008-12-17</span> </div> </li> <li> <div> <b>3. </b><a class="enjiyixqcontent" href="/patent-detail/06130429321298.html">STRAIN OF MYCELIAL MUSHROOM PENICILLIUM VERRUCULOSUM - PRODUCER OF COMPLEX OF CELLULASE, XYLANASE AND XYLOGLUCANASE AND WAY OF RECEPTION OF FERMENTAL PREPARATION OF COMPLEX OF CELLULASE, XYLANASE AND XYLOGLUCANASE FOR HYDROLYSIS OF CELLULOSE AND HEMICELLULOSE</a> <b>[P]</b> . <span> 外国专利：  RU2361918C1 </span> <span> . 2009-07-20</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：菌丝菌青霉菌-纤维素酶，木聚糖酶和木葡糖苷酶复合物的生产者，以及发酵制备纤维素酶，木聚糖酶和木葡糖苷酶复合物的方法，用于纤维素和纤维素的水解 </span> </p> </li> <li> <div> <b>4. </b><a class="enjiyixqcontent" href="/patent-detail/06130411550891.html">CELLULASE MIXTURES OPTIMIZED FOR DIFFERENTIALLY PRETREATED LIGNOCELLULOSE AND METHOD FOR PREPARING THE SAME</a> <b>[P]</b> . <span> 外国专利：  KR20160005870A </span> <span> . 2016-01-18</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：针对不同预处理的木质纤维素进行优化的纤维素酶混合物及其制备方法 </span> </p> </li> <li> <div> <b>5. </b><a class="enjiyixqcontent" href="/patent-detail/06130411391521.html">Cellulase mixtures optimized for differentially pretreated lignocellulose and method for preparing the same</a> <b>[P]</b> . <span> 外国专利：  KR101617222B1 </span> <span> . 2016-05-03</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：针对差异预处理木质纤维素进行了优化的纤维素酶混合物及其制备方法 </span> </p> </li> </ul> </div> </div> </div> <div class="theme cardcommon" style="overflow: auto;display:none"> <h3 class="all_title" id="enpatent55">相关主题</h3> 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