Metabolic strategies for microbial glycerol overproduction

Lim Chanyuen; Lin Annette Luhe; Zhao Hua

摘要

Abstract > Glycerol as a platform chemical has wide applications in chemical, pharmaceutical, and food industries and is a useful feedstock for the production of bio‐based chemicals. There is an increasing demand for sustainable glycerol production and microbial glycerol production is a promising alternative. The paper highlighted this new trend and summarized recent research progress on microbial glycerol production, and focused mainly on the efforts achieved in rational design of <fi>S. cerevisiae</fi> , as well as <fi>Escherichia coli</fi> , to overcome the limitations of glycerol production in these two microorganisms. More efforts are required for overcoming hurdles in yield and productivity enhancement for large scale production. ? 2017 Society of Chemical Industry </abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> <div class="translation abstracttxt"> <span class="zhankaihshouqi fivelineshidden" id="abstract"> <span>机译：</span><abstract xmlns =“http://www.wiley.com/namespaces/wiley”type =“main”xml：lang =“en”> <title type =“main”>抽象</ title> >甘油平台化学品在化学，制药和食品工业中具有广泛的应用，是生产生物基化学品的有用原料。对可持续甘油生产的需求越来越大，微生物甘油生产是一个有前途的替代品。本文突出了这种新趋势，总结了近期微生物甘油生产的研究进展，主要集中在为<FI> S的合理设计中实现的努力。酿酒酵母</ fi>，以及<fi>大肠杆菌</ fi>，克服甘油生产在这两种微生物中的局限性。克服更多努力克服障碍以获得大规模生产的产量和生产力增强。还 2017化学工业学会</ p> </摘要> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> </div> <div class="record"> <h2 class="all_title" id="enpatent33" >著录项</h2> <ul> <li> <span class="lefttit">来源</span> <div style="width: 86%;vertical-align: text-top;display: inline-block;"> <a href='/journal-foreign-33908/'>《Journal of Chemical Technology & Biotechnology》</a> <b style="margin: 0 2px;">|</b><span>2018年第3期</span><b style="margin: 0 2px;">|</b><span>共5页</span> </div> </li> <li> <div class="author"> <span class="lefttit">作者</span> <p id="fAuthorthree" class="threelineshidden zhankaihshouqi"> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Lim Chanyuen&option=202" target="_blank" rel="nofollow">Lim Chanyuen;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Lin Annette Luhe&option=202" target="_blank" rel="nofollow">Lin Annette Luhe;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Zhao Hua&option=202" target="_blank" rel="nofollow">Zhao Hua;</a> </p> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zkzz" style="display: none;">展开▼</span> </div> </li> <li> <div style="display: flex;"> <span class="lefttit">作者单位</span> <div style="position: relative;margin-left: 3px;max-width: 639px;"> <div class="threelineshidden zhankaihshouqi" id="fOrgthree"> <p>Agency for ScienceTechnology and Research (A*STAR)Jurong Island Singapore;</p> <p>Agency for ScienceTechnology and Research (A*STAR)Jurong Island Singapore;</p> <p>Agency for ScienceTechnology and Research (A*STAR)Jurong Island Singapore;</p> </div> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zhdw" style="display: none;">展开▼</span> </div> </div> </li> <li > <span class="lefttit">收录信息</span> <span style="width: 86%;vertical-align: text-top;display: inline-block;"></span> </li> <li> <span class="lefttit">原文格式</span> <span>PDF</span> </li> <li> <span class="lefttit">正文语种</span> <span>eng</span> </li> <li> <span class="lefttit">中图分类</span> <span><a href="https://www.zhangqiaokeyan.com/clc/180.html" title="生物工程学（生物技术）">生物工程学（生物技术）;</a><a href="https://www.zhangqiaokeyan.com/clc/20827.html" title="生物化学过程">生物化学过程;</a></span> </li> <li class="antistop"> <span class="lefttit">关键词</span> <p style="width: 86%;vertical-align: text-top;"> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=metabolic strategies&option=203" rel="nofollow">metabolic strategies;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=glycerol fermentation&option=203" rel="nofollow">glycerol fermentation;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=saccharomyces cerevisiae&option=203" rel="nofollow">saccharomyces cerevisiae;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=escherichia coli&option=203" rel="nofollow">escherichia coli;</a> </p> <div class="translation"> 机译：代谢策略;甘油发酵;酿酒酵母;大肠杆菌; </div> </li> </ul> </div> </div> <div class="literature cardcommon"> <div class="similarity "> <h3 class="all_title" id="enpatent66">相似文献</h3> <div class="similaritytab clearfix"> <ul> <li class="active" >外文文献</li> <li >中文文献</li> <li >专利</li> </ul> </div> <div class="similarity_details"> <ul > <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/journal-foreign-detail/0704025422816.html">Metabolic strategies for microbial glycerol overproduction</a> <b>[J]</b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Lim Chanyuen&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Lim Chanyuen,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Lin Annette Luhe&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Lin Annette Luhe,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Zhao Hua&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Zhao Hua </a> <a href="/journal-foreign-33908/" target="_blank" rel="nofollow" class="tuijian_authcolor">Journal of Chemical Technology & Biotechnology .</a> <span>2018</span><span>,第3期</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：微生物甘油过量生产的代谢策略</span> </p> </li> <li> <div> <b>2. </b><a class="enjiyixqcontent" href="/journal-foreign-detail/0704027921403.html">Achieving efficient nitrite accumulation in glycerol-driven partial denitrification system: Insights of influencing factors, shift of microbial community and metabolic function</a> <b>[J]</b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Zhang Teng&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Zhang Teng,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Cao Jiashun&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Cao Jiashun,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Zhang Yilei&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Zhang Yilei,</a> <a href="/journal-foreign-14911/" target="_blank" rel="nofollow" class="tuijian_authcolor">Bioresource Technology: Biomass, Bioenergy, Biowastes, Conversion Technologies, Biotransformations, Production Technologies .</a> <span>2020</span><span>,第1期</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：在甘油驱动的部分反硝化系统中实现高效的亚硝酸盐积累：影响因素，微生物群落变化和代谢功能的见解</span> </p> </li> <li> <div> <b>3. </b><a class="enjiyixqcontent" href="/journal-foreign-detail/0704027921723.html">Achieving efficient nitrite accumulation in glycerol-driven partial denitrification system: Insights of influencing factors, shift of microbial community and metabolic function</a> <b>[J]</b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Zhang Teng&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Zhang Teng,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Cao Jiashun&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Cao Jiashun,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Zhang Yilei&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Zhang Yilei,</a> <a href="/journal-foreign-14911/" target="_blank" rel="nofollow" class="tuijian_authcolor">Bioresource Technology: Biomass, Bioenergy, Biowastes, Conversion Technologies, Biotransformations, Production Technologies .</a> <span>2020</span><span>,第1期</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：在甘油驱动的部分反硝化系统中实现高效的亚硝酸盐积累：影响因素，微生物群落变化和代谢功能的见解</span> </p> </li> <li> <div> <b>4. </b><a class="enjiyixqcontent" href="/academic-conference-foreign_10th-international-conference-chemical-process-engineering_thesis/02051506457.html">Novel Strategies for Overproduction of Microbial Products</a> <b>[C]</b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Sheetal Raina&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Sheetal Raina,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Tania Murphy&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Tania Murphy,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Daniela De Vizio&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Daniela De Vizio,</a> <a href="/conference-foreign-7990/" target="_blank" rel="nofollow" class="tuijian_authcolor">10th international conference on chemical and process engineering .</a> <span>2011</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：微生物产品超量生产的新策略</span> </p> </li> <li> <div> <b>5. </b><a class="enjiyixqcontent" href="/academic-degree-foreign_mphd_thesis/0206157572.html">Quantitative analysis of metabolic pathways in Catharanthus roseus hairy roots metabolically engineered for terpenoid indole alkaloid overproduction.</a> <b>[D] </b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Sander, Guy William, II.&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Sander, Guy William, II. </a> <span>2009</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：代谢工程化用于萜类吲哚生物碱过量生产的长春花毛状根代谢途径的定量分析。</span> </p> </li> <li> <div> <b>6. </b><a class="enjiyixqcontent" href="/academic-journal-foreign-pmc_detail_thesis/040006278111.html">Feeding Strategies of Two-Stage Fed-Batch Cultivation Processes for Microbial Lipid Production from Sugarcane Top Hydrolysate and Crude Glycerol by the Oleaginous Red Yeast Rhodosporidiobolus fluvialis</a> <b>[O] </b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Rujiralai Poontawee&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Rujiralai Poontawee,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Savitree Limtong&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Savitree Limtong </a> <span>2020</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：产油红酵母红曲霉菌发酵甘蔗粗水解物和粗甘油生产微生物的两阶段补料分批培养的供料策略</span> </p> </li> <li> <div> <b>7. </b><a class="enjiyixqcontent" href="/open-access_resources_thesis/01000126501126.html">Feeding Strategies of Two-Stage Fed-Batch Cultivation Processes for Microbial Lipid Production from Sugarcane Top Hydrolysate and Crude Glycerol by the Oleaginous Red Yeast Rhodosporidiobolus fluvialis</a> <b>[O] </b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Savitree Limtong&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Savitree Limtong,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Rujiralai Poontawee&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Rujiralai Poontawee </a> <span>2020</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：从甘蔗顶水解产物和粗甘油的微生物脂质生产的两阶段喂养培养工艺的饲养策略用含红玉米氏菌罗达孢子毒素氟尿嘧啶</span> </p> </li> </ul> <ul style="display: none;"> <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/academic-journal-cn_biological-process_thesis/0201264180342.html">代谢工程大肠杆菌过量生产番茄红素</a> <b>[J]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=翁志明1&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 翁志明1</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=王玥1&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,王玥1</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=刘建忠1&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,刘建忠1</a> <span> <a href="/journal-cn-38855/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 生物过程 </a> </span> <span> . 2012</span><span>,第002期</span> </span> </div> </li> <li> <div> <b>2. </b><a class="enjiyixqcontent" href="/academic-journal-cn_international-journal-stomatology_thesis/0201238366002.html">微生物代谢组学的研究策略及其在人体微生物中的应用</a> <b>[J]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=余培&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 余培</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=薛晶&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,薛晶</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=李伟&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,李伟</a> <span> <a href="/journal-cn-6295/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 国际口腔医学杂志 </a> </span> <span> . 2015</span><span>,第006期</span> </span> </div> </li> <li> <div> <b>3. </b><a class="enjiyixqcontent" href="/academic-journal-cn_jiangsu-agricultural-sciences_thesis/020122998747.html">微生物法生产甘油酸发酵培养基的优化</a> <b>[J]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=姚浩轲&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 姚浩轲</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=方亚坤&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,方亚坤</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=周配&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,周配</a> <span> <a href="/journal-cn-7304/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 江苏农业科学 </a> </span> <span> . 2018</span><span>,第003期</span> </span> </div> </li> <li> <div> <b>4. </b><a class="enjiyixqcontent" href="/academic-journal-cn_bulletin-fermentation-science-technology_thesis/0201246228438.html">微生物利用粗甘油生产增值产品的研究</a> <b>[J]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=杨猛&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 杨猛</a> <span> <a href="/journal-cn-9118/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 发酵科技通讯 </a> </span> <span> . 2014</span><span>,第001期</span> </span> </div> </li> <li> <div> <b>5. </b><a class="enjiyixqcontent" href="/academic-journal-cn_cereal-feed-industry_thesis/020122135639.html">椰子油和单月桂酸甘油酯对断奶仔猪生产性能及粪便微生物的影响</a> <b>[J]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=李涛&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 李涛</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=王春维&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,王春维</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=万志友&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,万志友</a> <span> <a href="/journal-cn-9366/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 粮食与饲料工业 </a> </span> <span> . 2013</span><span>,第006期</span> </span> </div> </li> <li> <div> <b>6. </b><a class="enjiyixqcontent" href="/academic-conference-cn_meeting-41661_thesis/020222492554.html">基于代谢工程改造和发酵调控策略强化微生物利用粗甘油合成2,3-丁二醇的能力</a> <b>[C]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=杨套伟&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 杨套伟</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=张显&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,张显</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=徐美娟&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,徐美娟</a> <span> <a href="/conference-cn-41661/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 中国生物工程学会第十届学术年会暨2016年全国生物技术大会 </a> <span> <span> . -1</span> </span> </div> </li> <li> <div> <b>7. </b><a class="enjiyixqcontent" href="/academic-degree-domestic_mphd_thesis/020313347966.html">一类具有增长抑制和代谢过量的微生物连续培养模型的研究</a> <b>[A] </b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=郭庆广&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 郭庆广</a> <span> . 2000</span> </span> </div> </li> </ul> <ul style="display: none;"> <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/patent-detail/061201555785.html">导入阿拉伯糖代谢途径的产木糖醇微生物及使用所述微生物生产木糖醇的方法</a> <b>[P]</b> . <span> 中国专利： CN102712894B </span> <span> . 2015.02.18</span> </div> </li> <li> <div> <b>2. </b><a class="enjiyixqcontent" href="/patent-detail/06120102590361.html">导入异源代谢途径的产1-丙醇微生物及使用所述微生物生产1-丙醇的方法</a> <b>[P]</b> . <span> 中国专利： CN103923871A </span> <span> . 2014-07-16</span> </div> </li> <li> <div> <b>3. </b><a class="enjiyixqcontent" href="/patent-detail/06130417230956.html">PRODUCTION METHOD FOR 1,3-PROPANEDIOL USING RECOMBINANT MICROBIAL STRAIN HAVING GLYCEROL OXIDATION METABOLIC PATHWAY BLOCKED</a> <b>[P]</b> . <span> 外国专利：  EP2407544A4 </span> <span> . 2014-01-22</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：具有甘油氧化代谢途径的重组微生物菌株制备1，3-丙二醇的方法 </span> </p> </li> <li> <div> <b>4. </b><a class="enjiyixqcontent" href="/patent-detail/06130422291796.html">PRODUCTION METHOD FOR 1,3-PROPANEDIOL USING RECOMBINANT MICROBIAL STRAIN HAVING GLYCEROL OXIDATION METABOLIC PATHWAY BLOCKED</a> <b>[P]</b> . <span> 外国专利：  EP2407544A1 </span> <span> . 2012-01-18</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：具有甘油氧化代谢途径的重组微生物菌株制备1，3-丙二醇的方法 </span> </p> </li> <li> <div> <b>5. </b><a class="enjiyixqcontent" href="/patent-detail/06130422291795.html">PRODUCTION METHOD FOR 1,3-PROPANEDIOL USING RECOMBINANT MICROBIAL STRAIN HAVING GLYCEROL OXIDATION METABOLIC PATHWAY BLOCKED</a> <b>[P]</b> . <span> 外国专利：  EP2407544A9 </span> <span> . 2012-05-23</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：具有甘油氧化代谢途径的重组微生物菌株制备1，3-丙二醇的方法 </span> </p> </li> </ul> </div> </div> </div> <div class="theme cardcommon" style="overflow: auto;display:none"> <h3 class="all_title" id="enpatent55">相关主题</h3> <ul id="subject"> </ul> </div> </div> </div> </div> <div class="right rightcon"> <div class="details_img cardcommon clearfix" style="margin-bottom: 10px;display:none;" > </div> </div> </div> <div id="thesis_get_original1" class="downloadBth" style="bottom: 19px;z-index: 999;" onclick="ywcd('0704025422816','4',7,2,1,'',this,24)" class="delivery" prompt="010401" title="通过人工服务将文献原文发送至邮箱" >获取原文</div> <div class="journalsub-pop-up" style="display: none"> <div class="journal-sub"> <h2>期刊订阅</h2> <img src="https://cdn.zhangqiaokeyan.com/img/loginclose.png" alt="关闭" onclick="$('.journalsub-pop-up').hide()"> <p class="pardon">抱歉，该期刊暂不可订阅，敬请期待！</p> <p class="current">目前支持订阅全部北京大学中文核心（2020）期刊目录。</p> <div style="display: flex;margin-top: 69px;justify-content: space-between;"> <div class="no-sub" onclick="$('.journalsub-pop-up').hide()">暂不订阅</div> <div class="other-sub" onclick="continueSub('from=pc-detail')">继续订阅其他期刊</div> </div> </div> </div> <div class="right_btn"> <ul> <li class="gouwuche">  </li> <li class="yijian"> <a href="javascript:void(0);" onclick="link_analysis('/mycenter/auth/complaint.html',this)" title="意见反馈">意见反馈</a> </li> <li class="top"> <a href="javascript:scrollTo(0,0);" title="回到顶部">回到顶部</a> </li> <li class="shouye"> <a href="/" title="首页">回到首页</a> </li> </ul> </div> <div class="xllindexfooter"> <div class="xllindexfootercenter"> <div class="xllindexfooterleft left" > <div class="xllindexfooterleftli"> <ul> <li><a href="/about.html" title="关于掌桥">关于掌桥</a></li> <li><a href="/help/helpmap.html" title="资源导航">资源导航</a></li> <li><a href="/help/helpguide.html" title="新手指南">新手指南</a></li> <li><a href="/help/helpcenter.html" title="常见问题">常见问题</a></li> <li><a href="/sitemap.html" title="网站地图">网站地图</a></li> <li><a href="/help/helpcenter.html?type=9" title="版权声明">版权声明</a></li> </ul> </div> <div class="xllindexfooterleft"> <p class="xllindexfooterlefteamil">客服邮箱：kefu@zhangqiaokeyan.com</p> <div class="xllindexfooterlefttcp"> <div class="xllindexfooterpoliceiimg"></div> <div class="xllindexfooterpoliceispan"> <span>京公网安备：11010802029741号 </span> <span>ICP备案号：<a href="https://beian.miit.gov.cn" rel="nofollow" target="_blank" title="ICP备案号">京ICP备15016152号-6</a></span> <span>六维联合信息科技 (北京) 有限公司©版权所有</span> </div> </div> </div> </div> <div class="xllindexfooterright left"> <ul> <li> <p style="font-weight: bold;">客服微信</p> <div></div> </li> <li> <p style="font-weight: bold;">服务号</p> <div></div> </li> </ul> </div> </div> </div> <span id="0704025422816down" data-source="7," data-out-id="mZyTjFeL+nFSAofZhZ6j4w==," data-f-source-id="7" data-title="Metabolic strategies for microbial glycerol overproduction" data-price="20" data-site-name="" data-transnum="24" style="display:none;"></span> <input type="hidden" value="4" id="sourcetype"> <input type="hidden" value="33908" id="journalid"> <input type="hidden" value="1" id="inyn_provide_service_level"> <input type="hidden" value="https://cdn.zhangqiaokeyan.com" id="imgcdn"> <input type="hidden" value="1" id="isdeatail"> <input type="hidden" value="" id="syyn_indexed_database"> <input type="hidden" value="" id="servicetype"> <input type="hidden" id="pagename" value="Metabolic strategies for microbial glycerol overproduction"/> <input type="hidden" value="thesis_get_original" id="pageIdentification"> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/jquery-1.12.4.js"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/common/lwlh_ajax.js"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/zq.js?v=5.7.8"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/common/common.js?v=5.7.8"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/jquery.cookie.js"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/top.js?v=5.7.8"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/common/tip.js?v=5.7.8"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/common/login.js?v=5.7.8"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/common/down.js?v=5.7.8"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/common/search.js?v=5.7.8"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/common/newmail.js?v=5.7.8"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/searchtype.js?v=5.7.8"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/user/regist.js?v=5.7.8"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/zxs_solor/detail.js?v=5.7.8"></script> <script type="text/javascript" src="https://www.zhangqiaokeyan.com/statistics/static/pagecollection.js"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/tj.js"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/common/sse.js?v=5.7.8"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/pushbaidu.js"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/common/history.js"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/util/cookie.js?v=5.7.8"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/weipu/weipu.js?v=5.7.8"></script> </body> <script> $(function(){ var weiPuStatus = getCookie('WeiPuStatus'); if(weiPuStatus){ tipWeiPuStatus(weiPuStatus); delCookie('WeiPuStatus'); } getFacetKeywordVoInId(); var sourcetype = $("#sourcetype").val(); var inyn_provide_service_level = $("#inyn_provide_service_level").val(); if((sourcetype ==1||sourcetype==4)&&inyn_provide_service_level!=4){ getJournal(); } }) </script> </html>