In situ and post-synthesis immobilization of enzymes on nanocrystalline MOF platforms to yield active biocatalysts

摘要

BACKGROUNDVery recently, metal-organic framework (MOF) materials have been postulated as emerging supports to achieve solid-state enzyme-contained biocatalysts. In this work, post-synthesis and in situ strategies to immobilize -glucosidase and laccase on different MOF materials were studied. The MOF-based supports, i.e. MIL-53(Al), NH2-MIL-53(Al) and Mg-MOF-74, were prepared under soft and sustainable conditions (room temperature and pH values compatible with enzymatic activity), allowing development of the in situ strategy.