Interacting proteins of the essential two‐component system YycFG in Bacillus subtilis Bacillus subtilis

摘要

Abstract Two‐component signal transduction systems (TCSs) play a major role in adaption and survival of microorganisms in a dynamic and sometimes dangerous environment. YycFG is an essential TCS for many Gram‐positive bacteria, such as Bacillus subtilis , which regulates many important biological processes. However, its functional essentiality remains largely unknown. Here, we report several YycFG interacting proteins through coimmunoprecipitation (Co‐IP) and mass spectrometry (MS) analyses. We engineered the B. subtilis genome by a knock‐in approach to express YycG with a C‐terminal Flag and YycF with an N‐terminal HA tag. Immunoprecipitated fractions using anti‐Flag or anti‐HA agarose were subjected to MS analyses. A total of 41 YycG interacting proteins and four YycF interacting proteins were identified, most of which are involved in cellular metabolic processes, including cell wall synthesis and modification. The interactions of YycG with AsnB and FabL, as examples, were further validated in vitro. This study provided a clue that YycFG may be directly involved in regulation of bacterial central metabolic pathways.