SYNTHESIS OF TRIAZOL C-n-RIBONUCLEOSIDE PHOSPHORAMIDITES USING beta-RIBOFURANOSYL-C-n-ACETYLENES FOR RNA CATALYSIS PROBING

摘要

Novel C4-linked triazol C-0-, C-1- and C-2-ribonucleoside phosphoramidites for RNA catalysis probing were synthesized from beta-ribofuranosyl-C-n-acetylenes (n = 0-2), which were efficiently prepared by fragmentation of tetrazoles derived from cyanophosphates. N-Pivaloyloxymethyl moiety was selected for the protection of triazole-N, whose properties under acidic and basic conditions were investigated.