Fedbatch Operation Using Clostridium acetobutylicum Suspension Culture as Biocatalyst for Enhancing Hydrogen Production

摘要

We demonstrated the feasibility fo fedbatch operation using Clostridium acetobutylicum suspension culture as a biocatalyst for the continuous production of hydrogen The optimum operationg pH and temperature of the currrent cultivation system fro hydrogen production were pH 6.0 and 37deg C respectively.The volumetric loading of the bioreactor for yhydrogen productgion can be as high as 650 mmol hydrogen/L culture with a yield at approximately 2.0 mol hydrogen/mol glucose.Acetate and butyrate at a concentration higher than 13 g/L significantly inhibited not only cell growth but also hydrogen production(i.e.specific hydrogen production rate).Acetate appears to be less toxic than butyrate to the hydrogen production process.While significantly inhibiting cell growth,acetate hardly affected hydrogen production Finally the factors limiting cultivation performance were discussed and possible strategies for enhancing the production of hydrogen were proposed.