Evaluation of the RAPIDEC (R) CARBA NP and beta-CARBA (R) tests for rapid detection of Carbapenemase-producing Enterobacteriaceae

摘要

The analytical performances of the RAPIDEC CARBA NP (R) (bioMerieux) and the beta-CARBA (R) (Bio-Rad) tests were evaluated for the rapid detection of any known type of carbapenemases in Enterobacteriaceae. An international collection of 149 enterobacterial isolates comprising 111 Carbapenemase-Producing Enterobacteriaceae (CPE) and 38 non-carbapenemase producers was used. CPE included 32 carbapenemase producers of class A (18 KPC-2,1 FRI-1, 5 SME and 8 IMI), 33 of class B (13 NDM, 11 VIM and 9 IMP) and 46 of class D (15 OXA-48, 14 OXA-181, 10 OXA-204, 3 OXA-232 and 4 OXA-162). The RAPIDEC CARBA NP (R) and the beta-CARBA (R) tests were performed in strict accordance to the manufacturer's instructions and results were read within 2 h and 30 min, respectively. RAPIDEC CARBA NP (R) detected 104/111 CPE isolates compared to 72/111 for the beta-CARBA (R) test. The overall sensitivity and specificity were 93.7% and 100%, respectively, for the RAPIDEC CARBA NP test, and 64.9% and 90%, respectively, for the beta-CARBA (R) test. The beta-CARBA (R) test failed to detect all the non-KPC class A carbapenemases (14/14) and most (24/31) of the OXA-48-like producers (OXA-162, OXA-181, OXA-204 and OXA-232), and detected 1/1 OXA-163 and 1/1 OXA-405 as carbapenemase producers whereas these enzymes are rather defined as non carbapenemases. RAPIDEC CARBA NP (R) test exhibited better performances than those of the beta-CARBA (R) test and confirmed to be a reliable tool for the detection of CPE, especially for OXA-48-like producers. (C) 2017 Elsevier Inc. All rights reserved.