method for rapid detection and identification of one or more living target microorganisms in a sample; device for rapid detection and identification of one or more living target microorganisms in a sample; and kit testing for rapid detection and identification of one or more living target microorganisms in a sample for use in the method.

摘要

abstract method for rapid detection and identification of one or more living target microorganisms in a sample; device for rapid detection and identification of one or more living target microorganisms in a sample; and test kit for rapid detection and identification of one or more living target microorganisms in a sample for use in the method The invention provides methods, devices and kits for rapid detection and identification of one or more living target microorganisms in a liquid sample or developed in plates containing solid nutrient media. The invention includes mixing one or more target microorganisms with one or more aptamers and / or one or more antibodies, each conjugated to a reporter compound and specific for a first site on one or more target microorganisms to form a mixture. the mixture is placed in a permeable membrane having immobilized on it one or more aptamers attached to an amine compound, and / or one or more antibodies, each specific for a second site on one or more target microorganisms or a site on the conjugated aptamer and / or conjugated antibody. A detection solution is added to the membrane and detection and identification of one or more target microorganisms is achieved in approximately one hour or less.