Realization of ~(19)F MRI oximetry method using perfluorodecalin

摘要

Objective To identify the technical aspects of the potential use of clinically approved perfluorodecalin (PFD, C_(10)F_(18)) for ~(19)F magnetic resonance imaging (MRI) oximetry method at high magnetic field 7.05 T. Materials and methods ~(19)F T_1 measurements were made on a set of PFD samples with different oxygen contents (0%, 21%, and 100%) at room (21 °C) and body temperature (37 °C). In vivo MRI studies were carried out on one healthy rat and two rats with C6 brain glioma. Results The selective excitation of the magnetically equivalent ~(19)F nuclei of CF2 groups of trans-isomer of PFD, which give a doublet at a frequency of about -140 ppm (in relation the chemical shift of trifluoroacetic acid, which is -76.55 ppm) should be done for correct implementation of ~(19)F MRI oximetry method. The amount of PFD equal to 30 μl is the optimal for obtaining reliable data on the measured T_1 values. In this case, the standard deviation of T_1 does not exceed 5%. In vivo MRI studies showed that the values of the partial pressure of oxygen ( pO_2) decrease from normal values of about 38 mmHg (healthy brain) to almost 0 mmHg at the last stage of tumor growth. Conclusion The study showed the feasibility of the successful application of PFD for ~(19)F MRI oximetry method.