Lipids composition of visceral fat in overweight and obese patients using ~1H NMR at 9.4T

摘要

The adipose tissue has big influence in the risk to develop multiple diseases. The fatty acids composition of this tissue could be related to various disorders like cancer and diabetes~(1,2). The purpose of this study is to investigate the fatty acids composition of visceral fat in overweight and obese patients by quantifying metabolites signals in H1 NMR and estimating the diunsaturated (DUFA), monounsaturated (MUFA) and saturated fatty acids (SFA), and compare those estimations with Gas chromatography analysis. Subjects and Methods: Visceral fat samples were collected from 10 patients that undergoing abdominal elective surgery. Those patients were classified in overweight and obese patients using the body mass index (BMI). Fatty Acids Methyl Esters (FAME) from the visceral fat were extracted3 and analyzed using a 9.4T MRS (Bruker Bruker Ultra-shield) and gas chromatography (GC) with a mass spectrometer (Agilent technologies). We calculated the DUFA, MUFA and SFA by using the equations bellow4. We also calculated the contribution of the fatty acid with 16 carbons and 18 carbons. The peaks are shown in Figure 1.