Estradiol and estrogen receptor alpha in the mPOA and MeA in dwarf hamster (Phodopus campbelli) fathers

摘要

E-2 and its alpha receptor (ER alpha) have an essential role in the regulation of maternal behavior. In dwarf hamster (Phodopus campbelli), E-2 facilitates the display of paternal care, and it is possible that ER alpha is part of the neuroendocrine mechanisms that regulate this behavior. The aim of this study was to analyze the influence of copulation, cohabitation with the pregnant mate and the presence of the pups on paternal behavior, circulating E-2 levels and the presence of ER alpha in the medial preoptic area (mPOA) and medial amygdala (MeA) in dwarf hamsters. Eight males were mated with intact females (IFs), 8 with tubally ligated females (TLFs) and 8 with ovariectomized females (OFs). In males mated with IFs, paternal behavior tests were performed after copulation, halfway through pregnancy and 24 h after the birth of their pups. Males mated with TLFs were subjected to paternal behavior tests at equivalent periods as the males mated with IFs. In males mated with OFs, paternal behavior tests were performed on days 1, 5 and 10 of cohabitation. After the last paternal behavior tests, blood samples were taken for quantification of E-2 by radioimmunoassay (RIA), and the brains were dissected to determine ER alpha immunoreactivity (ir) in the mPOA and MeA. Fathers mated with IFs had higher serum E-2 concentrations and more ER alpha-ir cells in the mPOA than those of males mated with TLFs and OFs. These results suggest that E-2 and its ER alpha may be associated with paternity in the dwarf hamster.