Detection of        <i >Helicobacter pyloriHelicobacter pylori        in stool samples of young children using real‐time polymerase chain reaction

Beer‐Davidson Gany; Hindiyeh Musa; Muhsen Khitam

摘要

Abstract <section numbered="no" xml:id="hel12450-sec-0001"> <title type="main">Background > The aims of this study were to develop and validate a multiplex real‐time polymerase chain reaction (q‐ <fc>PCR</fc> ) assay of Helicobacter pylori in stool samples of healthy children. Additionally, we determined the prevalence of clarithromycin resistance and cagA gene in H.?pylori ‐positive samples. </section> <section numbered="no" xml:id="hel12450-sec-0002"> <title type="main">Materials and methods > Archived stool samples from 188 children aged 6‐9?years and 272 samples of 92 infants aged 2‐18?months were tested for H.?pylori antigens using enzyme immunoassay ( <fc>EIA</fc> ). A multiplex q‐ <fc>PCR</fc> assay was designed to detect H.?pylori 16S <fc>rRNA</fc> and urease and the human <fc>RN</fc> ase P gene as an internal control. Kappa coefficient was calculated to assess the agreement between q‐ <fc>PCR</fc> and <fc>EIA</fc> . </section> <section numbered="no" xml:id="hel12450-sec-0003"> <title type="main">Results > Laboratory validation of the q‐ <fc>PCR</fc> assay using quantitated H.?pylori <fc>ATCC</fc> 43504 extracted <fc>DNA</fc> showed S‐shaped amplification curves for all genes; the limit of detection was 1 <fc>CFU</f </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> <div class="translation abstracttxt"> <span class="zhankaihshouqi fivelineshidden" id="abstract"> <span>机译：</span><Abstract Type =“Main”XML：ID =“Hel12450-Abs-0001”XML：Lang =“en”> <标题类型=“main”>抽象</ title> <sectouned =“no”xml：id = “hel12450-sec-0001”> <标题类型=“main”>背景</ title> >本研究的目的是开发和验证多重实时聚合酶链反应（q-<fc> pcr < / fc>）测定的幽门螺杆菌在健康儿童粪便样本中的幽门螺杆菌。另外，我们确定了含量的慢霉素抗性和 Caga </ i>基因的患病率 H.?泊尔氏植物 - 样品。 </ p> </ section> <secto numbyed =“no”xml：id =“hel12450-sec-0002”> <title type =“main”>材料和方法</ title> >从188的存档粪便样本6-9岁的儿童？年龄和272岁的92次婴儿样品为2-18岁的婴儿，用于使用酶免疫测定（<FC> EIA </ Fc>）对抗原进行抗原测试。设计了多重Q- <Fc> PCR </ FC>测定以检测 H.?丙棒（H.?丙棒）16S <FC> RNA </ I>和<I>脲酶</ I>和人类< I> <FC> RN </ FC> ASE P </ I>基因作为内部控制。计算Kappa系数以评估Q-<Fc> PCR </ Fc>和<FC> EIA>之间的协议。 </ p> </ section> <secto numbyed =“no”xml：id =“hel12450-sec-0003”> <标题类型=“main”>结果</ title> > Q- < Fc> PCR </ FC>测定使用定量的 H.?丙棒，ATCC> ATCC </ FC> 43504提取的<FC> DNA </ FC>显示了所有基因的S形扩增曲线;检测限为1 <FC> CFU </ F. </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> </div> <div class="record"> <h2 class="all_title" id="enpatent33" >著录项</h2> <ul> <li> <span class="lefttit">来源</span> <div style="width: 86%;vertical-align: text-top;display: inline-block;"> <a href='/journal-foreign-32409/'>《Helicobacter》</a> <b style="margin: 0 2px;">|</b><span>2018年第1期</span><b style="margin: 0 2px;">|</b><span>共11页</span> </div> </li> <li> <div class="author"> <span class="lefttit">作者</span> <p id="fAuthorthree" class="threelineshidden zhankaihshouqi"> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Beer‐Davidson Gany&option=202" target="_blank" rel="nofollow">Beer‐Davidson Gany;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Hindiyeh Musa&option=202" target="_blank" rel="nofollow">Hindiyeh Musa;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Muhsen Khitam&option=202" target="_blank" rel="nofollow">Muhsen Khitam;</a> </p> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zkzz" style="display: none;">展开▼</span> </div> </li> <li> <div style="display: flex;"> <span class="lefttit">作者单位</span> <div style="position: relative;margin-left: 3px;max-width: 639px;"> <div class="threelineshidden zhankaihshouqi" id="fOrgthree"> <p>Department of Epidemiology and Preventive MedicineTel Aviv UniversityTel Aviv Israel;</p> <p>Department of Epidemiology and Preventive MedicineTel Aviv UniversityTel Aviv Israel;</p> <p>Department of Epidemiology and Preventive MedicineTel Aviv UniversityTel Aviv Israel;</p> </div> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zhdw" style="display: none;">展开▼</span> </div> </div> </li> <li > <span class="lefttit">收录信息</span> <span style="width: 86%;vertical-align: text-top;display: inline-block;"></span> </li> <li> <span class="lefttit">原文格式</span> <span>PDF</span> </li> <li> <span class="lefttit">正文语种</span> <span>eng</span> </li> <li> <span class="lefttit">中图分类</span> <span><a href="https://www.zhangqiaokeyan.com/clc/183.html" title="微生物学">微生物学;</a></span> </li> <li class="antistop"> <span class="lefttit">关键词</span> <p style="width: 86%;vertical-align: text-top;"> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=children&option=203" rel="nofollow">children;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=cytotoxin‐associated gene A&option=203" rel="nofollow">cytotoxin‐associated gene A;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=enzyme immunoassay&option=203" rel="nofollow">enzyme immunoassay;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Helicobacter pylori&option=203" rel="nofollow">Helicobacter pylori;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=q‐ PCR&option=203" rel="nofollow">q‐ PCR;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=stool samples&option=203" rel="nofollow">stool samples;</a> </p> <div class="translation"> 机译：儿童;细胞毒素相关基因A;酶免疫测定;幽门螺杆菌;Q-PCR;粪便样本; </div> </li> </ul> </div> </div> <div class="literature cardcommon" id="literaturereference" style="display:none"> <div class="similarity "> <h3 class="all_title" id="enpatent111">引文网络</h3> <div class="referencetab clearfix"> <ul id="referencedaohang"> <li dataid="referenceul">参考文献</li> <li dataid="citationul">引证文献</li> <li dataid="commonreferenceul">共引文献</li> <li dataid="commoncitationul">同被引文献</li> <li dataid="tworeferenceul">二级参考文献</li> <li dataid="twocitationul">二级引证文献</li> </ul> </div> <div class="reference_details" id="referenceList"> <ul id="referenceul"></ul> <ul id="citationul"></ul> <ul id="commonreferenceul"></ul> <ul id="commoncitationul"></ul> <ul id="tworeferenceul"></ul> <ul id="twocitationul"></ul> </div> </div> </div> <div class="literature cardcommon"> <div class="similarity "> <h3 class="all_title" id="enpatent66">相似文献</h3> <div class="similaritytab clearfix"> <ul> <li class="active" >外文文献</li> <li >中文文献</li> <li >专利</li> </ul> </div> <div class="similarity_details"> <ul > <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/journal-foreign-detail/0704025099498.html">Detection of <i >Helicobacter pyloriHelicobacter pylori in stool samples of young children using real‐time polymerase chain reaction</a> <b>[J]</b> . <span> <a 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Detection of Helicobacter pyloriHelicobacter pylori in stool samples of young children using real‐time polymerase chain reaction

摘要