Drug Discovery Applications of High-throughput Cytological Discovery Systems

摘要

Yokogawa's range of high-throughput cytological discovery systems includes the CellVoyager CV8000 high-throughput cytological discovery system, which is a large highend type, and the CQI confocal quantitative image cytometer, which is a small bench-top type, both of which use the CellPathJinder high content analysis (HCA) software. By using confocal scanning technologies based on microlens-enhanced Nipkow disks, both systems can automatically acquire a large amount of high-definition, 2D/3D confocal microscopic images at high speed and quantify the information contained in these images. These systems can also provide ideal culture conditions for cells through temperature control, CO_2 control, and a humidifier. These useful functions enable the systems to analyze various cellular responses and thus to be increasingly used in the drug discovery field. This paper introduces three examples of using these systems for drug discovery research: hepatotoxicity assay, cellular migration assay, and in vivo tumor metastasis assay using zebrafish.