A rapid method for establishment of a reverse genetics system for canine parvovirus

Yongle Yu; Jun Su; Jigui Wang; Ji Xi; Yaping Mao; Qiang Hou; Xiaomei Zhang; Weiquan Liu

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A rapid method for establishment of a reverse genetics system for canine parvovirus

机译：建立犬剖腹管病毒反向遗传系统的快速方法

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摘要

Abstract Canine parvovirus (CPV) is an important and highly prevalent pathogen of dogs that causes acute hemorrhagic enteritis disease. Here, we describe a rapid method for the construction and characterization of a full-length infectious clone (rCPV) of CPV. Feline kidney (F81) cells were transfected with rCPV incorporating an engineered EcoR I site that served as a genetic marker. The rescued virus was indistinguishable from that of wild-type virus in its biological properties.

机译：摘要犬剖视病毒（CPV）是犬的重要且高度普遍的病原体，导致急性出血性肠炎疾病。在这里，我们描述了一种快速的CPV构建和表征CPV的全长传染克隆（RCPV）。用RCPV转染翅膀的肾脏（F81）细胞，其包含作为遗传标记的工程化EcoR I位点。救助的病毒在其生物学特性中与野生型病毒的病毒无法区分。

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来源
《Virus Genes》 |2017年第6期|共7页
作者
Yongle Yu; Jun Su; Jigui Wang; Ji Xi; Yaping Mao; Qiang Hou; Xiaomei Zhang; Weiquan Liu;
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作者单位

State Key Laboratory of Agrobiotechnology Department of Biochemistry and Molecular Biology;

State Key Laboratory of Agrobiotechnology Department of Biochemistry and Molecular Biology;

State Key Laboratory of Agrobiotechnology Department of Biochemistry and Molecular Biology;

State Key Laboratory of Agrobiotechnology Department of Biochemistry and Molecular Biology;

State Key Laboratory of Agrobiotechnology Department of Biochemistry and Molecular Biology;

State Key Laboratory of Agrobiotechnology Department of Biochemistry and Molecular Biology;

State Key Laboratory of Agrobiotechnology Department of Biochemistry and Molecular Biology;

State Key Laboratory of Agrobiotechnology Department of Biochemistry and Molecular Biology;

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原文格式 PDF
正文语种 eng
中图分类医学微生物学（病原细菌学、病原微生物学）;
关键词
Canine parvovirus; Infectious clone; Genetic marker; Rescue virus; Biological properties; Reverse genetics;

机译：犬剖视病毒;传染性克隆;遗传标记;救援病毒;生物学特性;反向遗传;

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专利

1. A rapid method for establishment of a reverse genetics system for canine parvovirus [J] . Yongle Yu, Jun Su, Jigui Wang, Virus Genes . 2017,第6期

机译：建立犬剖腹管病毒反向遗传系统的快速方法
2. Cloning of the canine RNA polymerase I promoter and establishment of reverse genetics for influenza A and B in MDCK cells [J] . Zhaoti Wang, Gregory M Duke Virology Journal . 2007,第1期

机译：犬RNA聚合酶I启动子的克隆以及MDCK细胞中甲型和乙型流感的反向遗传学的建立
3. Establishment of reverse transcription loop-mediated isothermal amplification for rapid detection and differentiation of canine distemper virus infected and vaccinated animals [J] . Liu Da-Fei, Liu Chun-Guo, Tian Jin, Infection, Genetics and Evolution: Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases . 2015,第Null期

机译：建立逆转录环介导的等温扩增，以快速检测和区分犬瘟热病毒感染和接种的动物
4. A rapid prototyping methodology for reverse engineering of legacy electronic systems [C] . Deno, S., Landis, . 1999

机译：用于旧电子系统的反向工程的快速原型方法
5. Molecular studies in two viral systems: I) Mechanisms of immune evasion in New World arenaviruses. II) Establishment of reverse genetics for Human rhinovirus C [D] . Fan, Lina 2010

机译：在两个病毒系统中的分子研究：I）新大陆芳烃病毒中逃避免疫的机制。 II）建立人类鼻病毒C的反向遗传学
6. Establishment of a Simple and Efficient Reverse Genetics System for Canine Adenoviruses Using Bacterial Artificial Chromosomes [O] . Hiromichi Matsugo, Tomoya Kobayashi-Kitamura, Haruhiko Kamiki, 2020

机译：使用细菌人工染色体建立一种犬腺病毒简单有效的逆向遗传体系
7. Cloning of the canine RNA polymerase I promoter and establishment of reverse genetics for influenza A and B in MDCK cells [O] . Duke Gregory M, Wang Zhaoti 2007

机译：犬RNA聚合酶I启动子的克隆以及MDCK细胞中甲型和乙型流感的反向遗传学的建立

A rapid method for establishment of a reverse genetics system for canine parvovirus

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