Failure of lipopolysaccharides to directly trigger the chemiluminescence response of isolated equine polymorphonuclear leukocytes.

摘要

The effects of lipopolysaccharides (LPS) on activation of equine polymorphonuclear leukocytes (PMN) were examined using chemiluminescence (CL). PMN were isolated from the whole blood on a discontinuous density gradient of Percoll. CL of 1.25 X 106 cells was measured after addition of Escherichia coli LPS (0.001-10 鎔/ml) alone or after incubation in autologous plasma for 1 h at 37鳦. Random variations of CL were observed after direct stimulation with LPS in 16 horses. CL of PMN increased in a dose-dependent fashion after incubation of LPS in plasma. Heat inactivation of plasma at 56鳦 for 1 h abolished this stimulatory effect. It is concluded that complement fragments or LPS ligands, such as LPS binding protein, may play a key role in activating PMN rather than cytokines.