The role of ethylene in abscission of sweet orange promoted by exogenous application of the abscission agent 5-chloro-3-methyl-4-nitro-1H-pyrazole (CMNP)

摘要

Mature 'Valencia' fruit were treated with 5-chloro-3-methyl-4-nitro-1H-pyrazole (CMNP), and the ethylene inhibitors aminoethoxy vinyl glycine (AVG) and silver thiosulfate (STS), to assess involvement of ethylene in abscission via CMNP. Fruit detachment force (FDF) and ethylene evolution were measured over time. Accumulation of the ethylene precursor 1-aminocyclopropane-1-carboxylate (ACC) in peel upon CMNP application was assessed and impact of application of ACC on FDF was estimated when applied 1 cm away from the abscission zone (AZ), to determine its potential as a signaling molecule in promoting fruit loosening. CMNP when applied alone reduced FDF by 50%. AVG and STS inhibited fruit loosening considerably at 500 mu M and 10 mM, respectively, when applied with 2 mM CMNP. ACC accumulation in peel was highest 2 days after CMNP application, which preceded the maximum decline in FDF which occurred a day later, indicating that ACC serves as a potential signaling molecule that triggers abscission in the AZ. The results of the study support the hypothesis that abscission via CMNP application is at least partly ethylene dependent. However, the decline in FDF by ACC was less than 50% of control which suggested involvement of another pathway in abscission via CMNP in sweet orange.