Proteomic Analysis of Exosomes and Its Application in HIV‐1 Infection

摘要

Abstract Exosomes are 30–100?nm extracellular vesicles secreted from late endosomes by various types of cells. Numerous studies have suggested that exosomes play significant roles in human immunodeficiency virus 1 (HIV‐1) biogenesis. Proteomics coupled with exosome fractionation has been successfully used to identify various exosomal proteins and helped to uncover the interactions between exosomes and HIV‐1. To inform the current progress in the intersection of exosome, proteomics, and HIV‐1, this review is focused on: i) analyzing different exosome isolation, purification methods, and their implications in HIV‐1 studies; ii) evaluating the roles of various proteomic techniques in defining exosomal contents; iii) discussing the research and clinical applications of proteomics and exosome in HIV‐1 biology.