TANDEM-PAIRED COLUMN CHEMISTRY FOR HIGH-THROUGHPUT PROTEOMIC EXOSOME ANALYSIS

摘要

Compositions and methods for sample preparation and mass spectrometric analysis of peptide samples obtained from biological samples are provided. The compositions and methods include a tandem column system in which a trap column is in fluid contact with an analytical column such as, for example, a HPLC column. As analytes are eluted from the analytical column, they can be passed to a detector (e.g., a mass spectrometer) for peptide analysis.