Prevalence of naturally occurring antisperm antibodies (ASA) in blood serum and seminal plasma, and their relationship with cryopreserved semen quality and fertility of buffalo bulls

摘要

40% ASA, IgA and IgG in serum; > 20% ASA, IgA and IgG in seminal plasma) for further comparisons. The frozen-thawed semen was also evaluated for percent motility (total and progressive), concentration, viability, abnormality, HOST, acrosome integrity, acrosome reaction, CMPT and first FSCR. The percentage of ASA (37.1 ±3.8 vs 22.3±1.5), IgA- (44.2±3.1 vs 21.6±2.7) and IgG-bound spermatozoa (35.5±3.0 vs 19.7±4.6) were significantly higher in serum than in seminal plasma. In blood serum, sperm motility, acrosome reaction, CMPT and FSCR were significantly higher in bulls with < 40% ASA as compared to their counterparts. In seminal plasma, bulls with < 20% ASA exhibited significantly higher sperm concentration as compared to their contemporary mates. Sperm concentration (982.2±50.4 vs 874.9±36.3%) in blood serum and acrosome reaction (56.6±2.7 vs 49.9±2.3%) in seminal plasma were the only parameters that were significantly higher in bulls with < 40% and < 20% IgA, respectively than in their counterparts. For IgG-type, sperm concentration and acrosome reaction were significantly higher in bulls with < 40% than in those with > 40% IgG in blood serum. In seminal plasma, acrosome reaction and FSCR were significantly higher in bulls with < 20% IgG than in their contemporary mates. In conclusion, detection of surface-bound ASA was associated with changes in quality and fertility of buffalo bull semen.]]>