RNA interference-based silencing of the alpha-amylase (amy1) gene in Aspergillus flavus decreases fungal growth and aflatoxin production in maize kernels

Gilbert Matthew K.; Majumdar Rajtilak; Rajasekaran Kanniah; Chen Zhi-Yuan; Wei Qijian; Sickler Christine M.; Lebar Matthew D.; Cary Jeffrey W.; Frame Bronwyn R.; Wang Kan

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RNA interference-based silencing of the alpha-amylase (amy1) gene in Aspergillus flavus decreases fungal growth and aflatoxin production in maize kernels

机译：基于RNA干扰的α-淀粉酶（AMY1）基因的沉默在玉米菌血管中的α-淀粉酶（AMY1）基因降低了玉米核中的真菌生长和黄曲霉毒素产生

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Main conclusion Expressing an RNAi construct in maize kernels that targets the gene for alpha-amylase in Aspergillus flavus resulted in suppression of alpha-amylase (amy1) gene expression and decreased fungal growth during in situ infection resulting in decreased aflatoxin production.

机译：主要结论在玉米核中表达RNAi构建体，其靶向α-淀粉酶的基因，导致α-淀粉酶（AMY1）基因表达抑制，降低原位感染期间的真菌生长导致降低的黄曲霉毒素产生。

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来源
《Planta: An International Journal of Plant Biology》 |2018年第6期|共9页
作者
Gilbert Matthew K.; Majumdar Rajtilak; Rajasekaran Kanniah; Chen Zhi-Yuan; Wei Qijian; Sickler Christine M.; Lebar Matthew D.; Cary Jeffrey W.; Frame Bronwyn R.; Wang Kan;
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作者单位

ARS Food &

Feed Safety Unit USDA 100 Robert E Lee Blvd New Orleans LA 70124 USA;

ARS Food &

Feed Safety Unit USDA 100 Robert E Lee Blvd New Orleans LA 70124 USA;

ARS Food &

Feed Safety Unit USDA 100 Robert E Lee Blvd New Orleans LA 70124 USA;

Louisiana State Univ Agr Ctr Dept Plant Pathol &

Crop Physiol 302 Life Sci Bldg Baton Rouge LA 70803 USA;

ARS Food &

Feed Safety Unit USDA 100 Robert E Lee Blvd New Orleans LA 70124 USA;

ARS Food &

Feed Safety Unit USDA 100 Robert E Lee Blvd New Orleans LA 70124 USA;

ARS Food &

Feed Safety Unit USDA 100 Robert E Lee Blvd New Orleans LA 70124 USA;

ARS Food &

Feed Safety Unit USDA 100 Robert E Lee Blvd New Orleans LA 70124 USA;

Iowa State Univ Plant Transformat Facil G405 Agronomy Hall Ames IA 50011 USA;

Iowa State Univ Plant Transformat Facil G405 Agronomy Hall Ames IA 50011 USA;

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原文格式 PDF
正文语种 eng
中图分类植物学;
关键词
Host-induced gene silencing; Mycotoxin; RNAi; Secondary metabolite; siRNA; Zea mays;

机译：宿主诱导的基因沉默;霉菌毒素;RNAi;次级代谢物;siRNA;Zea Mays;

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专利

1. RNA interference-based silencing of the alpha-amylase (amy1) gene in Aspergillus flavus decreases fungal growth and aflatoxin production in maize kernels [J] . Gilbert Matthew K., Majumdar Rajtilak, Rajasekaran Kanniah, Planta: An International Journal of Plant Biology . 2018,第6期

机译：基于RNA干扰的α-淀粉酶（AMY1）基因的沉默在玉米菌血管中的α-淀粉酶（AMY1）基因降低了玉米核中的真菌生长和黄曲霉毒素产生
2. Control of Aspergillus flavus growth and aflatoxin production in transgenic maize kernels expressing a tachyplesin-derived synthetic peptide, AGM182 [J] . Rajasekaran Kanniah, Sayler Ronald J., Sickler Christine M., Plant Science: An International Journal of Experimental Plant Biology . 2018,第期

机译：转基因玉米核中表达Tachypless衍生的合成肽的转基因玉米核中的曲霉生长和黄曲霉毒素的控制，AGM182
3. The Aspergillus flavus Spermidine Synthase ( spds) Gene, Is Required for Normal Development, Aflatoxin Production, and Pathogenesis During Infection of Maize Kernels [J] . Rajtilak Majumdar, Matt Lebar, Brian Mack, Frontiers in Plant Science . 2018,第1期

机译：黄曲霉亚精idine合酶（ spds ）基因是玉米发育中正常发育，黄曲霉毒素产生和感染机理的必需条件
4. Study on the key environmental factors Aspergillus flavus growth and aflatoxin production [C] . Zhang Chushu, Zhao Qin, Feng Jianxiong, Asian Pacific Conference on Mechanical Components and Control Engineering . 2014

机译：曲霉病毒生长和黄曲霉毒素生产重点环境因素研究
5. Effect of modified atmosphere packaging on the growth and aflatoxin production by Aspergillus flavus and Aspergillus parasiticus under tropical environmental storage conditions. [D] . Ellis, William Otoo. 1993

机译：气调包装对热带环境储存条件下黄曲霉和寄生曲霉的生长和黄曲霉毒素产生的影响。
6. The Aspergillus flavus Spermidine Synthase (spds) Gene Is Required for Normal Development Aflatoxin Production and Pathogenesis During Infection of Maize Kernels [O] . Rajtilak Majumdar, Matt Lebar, Brian Mack, -1

机译：玉米仁感染过程中正常发育黄曲霉毒素的产生和发病机理需要曲霉黄曲霉亚精胺合酶（spds）基因。
7. Control of Aflatoxin Production of Aspergillus flavus and Aspergillus parasiticus Using RNA Silencing Technology by Targeting aflD (nor-1) Gene [O] . Naresh Magan, Ahmed M. Abdel-Hadi, Daniel P. Caley, 2011

机译：靶向aflD（nor-1）基因的RNA沉默技术控制黄曲霉黄曲霉和寄生曲霉黄曲霉毒素的生产
8. Studies on the Control of Fungal Contamination and Aflatoxin Production by Aspergillus Flavus Link in a Cereal Grain by the Combination Treatment of Heat and Irradiation [R] . Odamtten, G. T. 1986

机译：热和辐射联合处理控制谷物中黄曲霉菌引起的真菌污染和黄曲霉毒素的研究

RNA interference-based silencing of the alpha-amylase (amy1) gene in Aspergillus flavus decreases fungal growth and aflatoxin production in maize kernels

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