GsSNAP33, a novel Glycine soja SNAP25-type protein gene: Improvement of plant salt and drought tolerances in transgenic Arabidopsis thaliana]]>

摘要

AbstractThe N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) superfamily, specifically the SNAP25-type proteins and t-SNAREs, have been proposed to regulate cellular processes and plant resistance mechanisms. However, little is known about the role of SNAP25-type proteins in combating abiotic stresses, specifically in wild soybean. In the current study, the isolation and functional characterization of the putative synaptosomal-associated SNAP25-type protein geneGsSNAP33from wild soybean (Glycine soja) were performed.GsSNAP33has a molecular weight of 33,311?Da and comprises 300 amino acid residues along with Qb-Qc SNARE domains. Multiple sequence alignment revealed the highest similarity of the GsSNAP33 protein to GmSNAP33 (91%), VrSNAP33 (89%), PvSNAP33 (86%) and AtSNAP33 (63%). Phylogenetic studies revealed the abundance of SNAP33 proteins mostly in dicotyledons. Quantitative real-time PCR assays confirmed thatGsSNAP33expression can be induced by salt, alkali, ABA and PEG treatments and thatGsSNAP33is highly expressed in the pods, seeds and roots ofGlycine soja. Furthermore, the overexpression of theGsSNAP33gene in WTArabidopsis thalianaresulted in increased germination rates, greater root lengths, improved photosynthesis, lower electrolyte leakage, higher biomass production and up-regulated expression levels of various stress-responsive marker genes, includingKINI,COR15A,P5Cs,RAB18,RD29AandCOR47in transgenic lines compared with those in WT lines. Subcellular localization studies revealed that the GsSNAP33-eGFP fusion protein was localized to the plasma membrane, while eGFP was distributed throughout whole cytoplasm of onion epidermal cells. Collectively, our findings suggest thatGsSNAP33, a novel plasma membrane protein gene ofGlycine soja, might be involved in improving plant responses to salt and drought stresses inArabidopsis.Highlights?GsSNAP33 is a plasma membrane protein.?The expression level of GsSNAP33 gene is induced under salt, alkali, ABA and osmotic stress condition.?Overexpression of GsSNAP33 up-regulated expression levels ofKINI,COR15A,P5Cs,RAB18,RD29AandCOR47marker genes.?Overexpression of GsSNAP33 resulted in improved salt & drought tolerance.]]>