The Quantitation of 7 beta-Hydroxy-Epiandrosterone in the Plasma and Seminal Plasma of Men With Different Degrees of Fertility

摘要

7 beta-hydroxy-epiandrosterone (7 beta-OH-EpiA) is an endogenous androgen metabolite that has been shown to exert neuroprotective, anti-inflammatory and anti-estrogenic effects. However, to the best of our knowledge no information is available about this androgen steroid in relation to sperm quality. We analyzed 7 beta-OH-EpiA in plasma and seminal plasma using a newly developed isotope dilution ultra-high performance liquid chromatography - mass spectrometry method. Validation met the requirements of FDA guidelines. Levels of 7 beta-OH-EpiA were measured in 191 men with different degrees of infertility. One-way analysis of variance followed by multiple comparison and correlation analysis adjusted for age, BMI and abstinence time were performed to evaluate the relationships between this steroid and sperm quality. Concentrations of 7 beta-OH-EpiA in seminal plasma were significantly higher in severely infertile men in comparison with healthy men and slightly infertile men. The same trend was found when blood plasma was evaluated. Furthermore, plasma 7 beta-OH-EpiA negatively correlated with sperm concentration (-0.215; p0.01) and total count (-0.15; p0.05). Seminal 7 beta-OH-EpiA was negatively associated with motility (-0.26; p0.01), progressively motile spermatozoa (-0.233; p0.01) and nonprogressively motile spermatozoa (-0.188; p0.05). 7 beta-OH-EpiA is associated with lower sperm quality and deserves more research in that respect.