Low evolutionary risk of iprovalicarb resistance in Phytophthora infestans

摘要

Iprovalicarb is a carboxylic acid amide (CAA) fungicide, highly effective in the control of potato late blight, causing by Phytophthora infestans. Due to cross-resistance with other CAA fungicides and moderate resistance risk of P. capsici to iprovalicarb, the evolutionary risk of P. infestans resistance to this fungicide and the contribution of inherited genes and environmental effect was evaluated using a common garden experiment. The results showed that the ratio of heritability and plasticity of iprovalicarb in the seven populations equaled 1.0, indicating both inherited genes and environmental factors were essential to iprovalicarb sensitivity in P. infestans. The pairwise population differentiation determined by SSR loci (F-ST) between populations ranged from 0.007 to 0.133 and the overall F-ST was significantly higher than population differentiation in RGR (Q(ST)), suggesting constraining selection acting on iprovalicarb sensitivity. We also found a new indicator of growth rate inhibition (GRI) for fungicide sensitivity, which was negatively correlated to growth rate in the absence of iprovalicarb, indicating a trade-off between iprovalicarb resistance and pathogen's growth. The constraining selection plus a trade-off between GRI and growth rate revealed low risk of P. infestans evolving resistance to iprovalicarb.