Wnt Signaling-Related Osteokines at Rest and Following Plyometric Exercise in Prepubertal and Early Pubertal Boys and Girls

摘要

Purpose: This study examined osteokines related to Wnt signaling at rest and in response to plyometric exercise in 12 boys [10.2 (0.4) y] and 12 girls [10.5 (0.4) y]. Methods: One resting (preexercise) and 3 postexercise (5 min, 1 h, and 24 h) blood samples were analyzed for sclerostin, dickkopf-related protein 1 (DKK-1), osteoprotegerin (OPG), and receptor activator of nuclear factor kappa-beta ligand (RANKL). Results: Girls had higher resting sclerostin than boys [187.1 (40.1) vs 150.4 (36.4) pg.mL(-1), respectively; P = .02]. However, boys had higher DKK-1 [427.7 (142.3) vs 292.8 (48.0) pg.mL(-1), respectively; P = .02] and RANKL [3.9 (3.8) vs 1.0 (0.4) pg.mL(-1), respectively; P .01] than girls. In girls, sclerostin significantly decreased 5-minute and 1-hour postexercise (chi(2)= 12.7, P = .01), and RANKL significantly decreased 5-minute postexercise (chi(2 )= 19.1, P .01) and continued to decrease up to 24-hour postexercise, with large effect sizes. In boys, DKK-1 significantly decreased 1-hour postexercise and remained lower than preexercise 24-hour postexercise (chi(2)= 13.0, P = .01). OPG increased in both boys (chi(2)= 13.7, P .01) and girls (chi(2) = 11.4, P = .01), with boys having significantly higher OPG at 5-minute and 1-hour postexercise, whereas in girls, this increase was only seen 24-hour postexercise. Conclusion: Plyometric exercise induces an overall anabolic osteokine response favoring osteoblastogenesis over osteoclastogenesis in both boys and girls although the timeline and mechanism(s) may be different.