The collective influence of 1, 25-dihydroxyvitamin D 3 with physiological fluid shear stress on osteoblasts

摘要

Highlights ? This study reports the collective influences of 1, 25(OH) 2 D 3 and flow shear stress (FSS) on osteoblasts. ? FSS-induced NO release is 1, 25 (OH) 2 D 3 concentration dependent with a pattern of 0-FSS?>?1-FSS?>?10-FSS?>?100-FSS. ? FSS-induced PGE 2 release is 1, 25 (OH) 2 D 3 concentration dependent with a pattern of 100-FSS?>?10-FSS?>?0-FSS?≈?1-FSS. ? ALP release and expressions of OPN, OCN followed the pattern 100-FSS?>?10-FSS?>?1-FSS?≈?0-FSS. ? The potential mechanism is that 1, 25 (OH) 2 D 3 modulates the focal adhesion and cytoskeleton. Abstract 1, 25-dihydroxyvitamin D 3 (1, 25 (OH) 2 D 3 ) and mechanical stimuli in physiological environment contributes greatly to osteoporosis pathogenesis. Wide investigations have been conducted on how 1, 25-dihydroxyvitamin D 3 and mechanical stimuli separately impact osteoblasts. This study reports the collective influences of 1, 25-dihydroxyvitamin D 3 and flow shear stress (FSS) on biological functions of osteoblasts. 1, 25 (OH) 2 D 3 were prepared in various kinds of concentrations (0, 1, 10, 100?nmmol/L), while physiological fluid shear stress (12?dynes/cm 2 ) was produced by using a parallel-plate fluid flow system. 1, 25 (OH) 2 D 3 affects the responses of ROBs to FSS, including the inhibition of NO release and cell proliferation as well as the promotion of PGE 2 release and cell differentiation. These findings provide a possible mechanism by which 1, 25(OH) 2 D 3 influences osteoblasts’ responses to FSS, thus most probably providing guidance for the selection of 1, 25(OH) 2 D 3 concentration and mechanical loading in order to produce functional bone tissues in vitro .