The present invention relates to the field of micro-fluidic chips. Disclosed are a fluid shear stress generation device and a fluid shear stress generation method. The device comprises a device body; a main flow passage and at least two branch flow passages are provided on the device body; the two ends of the main flow passage are provided with a fluid inlet and a main flow passage fluid outlet; one end of each branch flow passage is communicated with the main flow passage, and the other end is provided with a branch flow passage fluid outlet; valves capable of regulating cross sectional areas in the branch flow passages to allow fluid to pass through are provided in the branch flow passages. According to the present invention, by means of the cooperation of the main flow passage, the branch flow passages, and the valves, dynamic changes of the magnitudes and ratio of fluid shear stresses can be achieved without changing the flow velocity of input fluid and the structure of the device. In addition, the present invention can greatly expand the range of the ratio of the fluid shear stresses in first and final branch flow passages; moreover, the ratio at any point in the range can be covered. The device is simple in structure, and easy to popularize and implement.
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