New furostanol saponins with anti-inflammatory and cytotoxic activities from the rhizomes of Smilax davidiana

摘要

Graphical abstract Display Omitted Highlights ? Seven new furostanol glycosides were isolated from the ethanol extract of Smilax davidiana . ? The structures of new compounds were determined on the basis of NMR spectroscopy and HR-ESI-MS. ? Compounds 3 , 5 and 7 were found to have modest anti-inflammatory effects. ? Davidianoside F ( 6 ) showed activity against MCF-7 and HELA cell lines. Abstract Seven new furostanol saponins have been isolated from the rhizomes of Smilax davidiana . Their structures were established by 2D NMR spectroscopic techniques ( 1 H, 1 H-COSY, NOESY, HSQC and HMBC), mass spectrometry and comparison with the literature. The isolated compounds were subjected to evaluate anti-inflammatory and cytotoxic activities in vitro. Compounds 3 , 5 and 7 were found to have modest anti-inflammatory effects through suppression of IL-1β production and promote the expression of IL-10 in LPS-stimulated RAW 264.7 cells. Davidianoside F ( 6 ) showed activity against MCF-7 and HELA cell lines at the concentration of 10.2μM and 4.3μM, respectively. ]]>