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The Rise of CRISPR/Cas for Genome Editing in Stem Cells

机译:CRISPR / CAS在干细胞中进行基因组编辑的兴起

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Genetic manipulation is a powerful tool to establish the causal relationship between a genetic lesion and a particular pathological phenotype. The rise of CRISPR/Cas9 genome-engineering tools overcame the traditional technical bottleneck for routine site-specific genetic manipulation in cells. To create the perfect in vitro cell model, there is significant interest from the stem cell research community to adopt this fast evolving technology. This review addresses this need directly by providing both the up-to-date biochemical rationale of CRISPR-mediated genome engineering and detailed practical guidelines for the design and execution of CRISPR experiments in cell models. Ultimately, this review will serve as a timely and comprehensive guide for this fast developing technology.
机译:遗传操作是一种强大的工具,可以建立遗传病变与特定病理表型之间的因果关系。 CRISPR / CAS9基因组工程的兴起克服了传统的技术瓶颈,用于细胞中的常规场所特异性遗传操作。 为了创造完美的体外细胞模型,对干细胞研究界具有显着兴趣,采用这种快速发展的技术。 通过提供CRISPR介导的基因组工程的最新生物化学理由以及细胞模型中CRISPR实验的详细实际指南,直接解决了这一点。 最终,本综述将作为这种快速发展技术的及时和全面的指导。

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