Validation study to use bio-PCR and seed extract PGR for pre-screening in the detection Xdntharxmampestrisw. am/jesteand pv. raphanii Bfdssica seed; supporting the proposal C.7.1 to modify Seed Health Method 7-019a

摘要

The performance of two optional pre-screening methods - bio-PCR and seed extract PCR - in the detection of Xanthomonas campestris pv. campestris and pv. raphani (Xcc) in untreated cabbage (Brassica sp.) seeds was evaluated.As with dilution plating, bio-PCR and seed extract PCR showed a 100 per cent sensitivity in the comparative test. Both bio-PCR and seed extract PCR show acceptable specificity and accuracy. Both methods show some possibility of false-positives, which isacceptable since these methods are suggested as a pre-screening method only. For positive samples both methods show high level accordance and concordance.Therefore, the results support the use of bio-PCR and seed extract PCR as pre-screening methods for ISTA 7-019a. It is thereforeproposed to include both bio-PCR and seed extract PCR as pre-screening methods in ISTA 7-019a.