The variation of berry development and DNA methylation after treatment with 5-azaC on 'Kyoho' grape

摘要

DNA methylation has been proved to function in fruit ripening. The different concentrations of azacitidine (5-azaC), an inhibitor of methyltransferase, were sprayed on 'Kyoho' grape berries to explore the changes of DNA methylation, the effect of 5-azaC on berry ripening, and the relationship between DNA methylation and reactive oxygen species (ROS) metabolism during the grape berry development. The results showed that the treatment of 100 mu M 5-azaC could promote the berry ripening of 'Kyoho' 20 days earlier than the control. The assessment of methylation by methylation sensitive amplification polymorphism (MSAP) markers after the treatment of 100 mu M 5-azaC, demonstrated that the methylation rate of the treatment was all lower than the control at any berry development periods. 5-azaC treatments influenced the ROS metabolic activities and related physiological indexes of berries. The sucrose and soluble solid content of 5-azaC-treated berries were significantly higher than control at 75 dpa. The change trend of H2O2 content of the control was similar with the 100 mu M 5-azaC treatment. All treatments enhanced rates of superoxide anion (O-2(-center dot)) production. Superoxide dismutase (SOD) activity in treated berries were higher than the control and had significant difference with the control at 35 dpa. The results demonstrated that 5-azaC could accelerate grape berries ripening, and there was a connection between ROS metabolism and DNA methylation during the grape berry development.