Effect of Tranilast on morphine-induced MCF-7 cell viability and death.

摘要

The anti-allergic drug Tranilast may induce cell line proliferation and prevent apoptosis. MCF-7 cells were grown in Dulbecco's modified eagle's medium, fetal bovine serum, non-essential amino acid, L-glutamine, penicillin and streptomycin in T-25 cm2tissue culture flasks. Cells were plated in 24 well culture plates with 104 cells/mL densities for 12h, further cultured in Tranilast media for 24h. Percentage viability was measured at 490-492nm by ELISA. Morphine was used as an apoptosis control. Oneday after plating, cells were washed with PBS. Effect of Tranilast on viability and cell death was determined by microscopy, trypan blue, Hoescht/PI staining and lactate dehydrogenase test. Data analyzed using one-way ANOVA followed by Tukey’s post hoctests suggested that Tranilast increased cell viability and decreased cell death (p<0.05).