Downregulation of SRC Kinase Signaling Inhibitor 1 (SRCIN1) Expression by MicroRNA-32 Promotes Proliferation and Epithelial-Mesenchymal Transition in Human Liver Cancer Cells

摘要

MicroRNAs play an important role in regulating gene expression by binding to the 3'-UTR of target mRNAs. In this study, we have made an attempt to assess the molecular mechanisms by which miR-32 suppresses the expression of SRCIN1, thereby leading to promotion of proliferation and epithelial-mesenchymal transition of human liver cancer cells. Human liver cancer cell line HepG2 was transfected with miR-32 mimics and its control. The HepG2 cells were assessed for miR-32 expression. The transfected cells were then studied for SRCIN1 expression by luciferase assay, effect of transfection on cell proliferation, and epithelial-mesenchymal transition. SRCIN1 expression was downregulated in the human liver cancer cell line HepG2. Overexpression of SRCIN I inhibited the proliferation of human liver HepG2 cancer cells and blocked epithelial-mesenchymal transition. It was observed that SRCIN1 expression was regulated by miR-32 in human liver cancer cells. Overexpression of miR-32 promoted cell proliferation and epithelial-mesenchymal transition of human liver cancer HepG2 cells. Our data demonstrated that SRCIN1 functions as a tumor suppressor in human liver cancers. Additionally, SRCIN1 functions to inhibit the proliferation and epithelial-mesenchymal transition of human liver cancer HepG2 cells. miRNA-32 was a direct target of SRCIN1. Overexpression of miR-32 promoted cell proliferation and epithelial-mesenchymal transition of human liver cancer HepG2 cells.