Instrumental Analysis on Optimization of Laccase Production from Pleurotus ostreatus strain PKN 04 in Different Substrate by Solid State Fermentation (SSF)

摘要

Solid state fermentation (SSF) is a green process to produce enzymes from fungi. Different substrates such as rice husk, paddy straw, sugarcane bagasse and wheat husk taken from top soil were used to produce laccase by Pleurotus ostreatus strain PKN 04. The process of SSF was characterized by FTIR. Microbial fermentation resulted in conversion of complex polymeric lignocelluloses substrates into simpler monomers. Fourier transform infrared spectroscopy (FT-IR) analyses of metabolites confirmed the breakdown and complete degradation of lignin, cellulose and polysaccharides at different fermentation time intervals of 0, IS and 30 days. The accessibility of enormous agro-industrial wastes from crop cultivation and food processing areas, offers a advantageous way to cultivate the bacteria and fungi that convert them into different Industrially important products. Production of lignocellulolytic enzymes using different plant raw materials by white rot fungi have successfully been performed in Submerged Fermentation (SmF) and Solid-State Fermentation (SSF) . Solid state fermentation is a process that commonly used to produce metabolites from the microbes especially from fungi. Laccases are N-glycosylated multi copper oxidases belonging to the group of the blue copper proteins". They can catalyze the oxidation of many substances coupled to the reduction of molecular oxygen to water.