The Use of HILIC Zwitterionic Phase Superficially Porous Particles for Metabolomics Analysis

摘要

Recently, superficially porous particles (SPPs) have generated significant interest because of the enhanced separation efficiency achieved at lower back pressure compared to that obtained using fully porous particles of the same particle size. Although many reversed-phase chemistries are available on SPPs, the chemistries for hydrophilic-interaction chromatography (HILIC) are limited. This article highlights the use of a new HILIC zwitterionic phase on superficially porous particles. A study on the use of a novel mobile-phase additive to achieve superior peak shape and isomer separation is also discussed, as well as improved liquid chromatography mass spectrometry (LC-MS) detection capabilities for metabolomics analysis.