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首页> 外文期刊>Molecular biology reports >Calcium hypochlorite on mouse embryonic fibroblast cells (NIH3T3) in vitro cytotoxicity and genotoxicity: MTT and comet assay
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Calcium hypochlorite on mouse embryonic fibroblast cells (NIH3T3) in vitro cytotoxicity and genotoxicity: MTT and comet assay

机译:在小鼠胚胎成纤维细胞(NIH3T3)上的次氯酸钙体外细胞毒性和遗传毒性:MTT和COMET测定

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摘要

Antimicrobial irrigation solutions are widely used under clinical settings. Their effect on dental tissue is a subject of recent research, which aims for a safer irrigant for clinical use. In this regard, here our goal was to evaluate the cytotoxicity and the genotoxicity ofcalcium hypochlorite(Ca(OCl)(2)) solution, along with NaOCl, on Mouse embryonic fibroblast cells (NIH3T3). First, Cells were treated either with NaOCl or Ca(OCl)(2)in a time- and dose-dependent manner for cytotoxicity by 3-(4,5-dimethylthiazolyl-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, then cell viability was calculated according to cell proliferation plots. Secondly, genotoxicity was assessed by Comet assay. Data were statistically analyzed by Tukey's test (P < .05). NaOCl and Ca(OCl)(2)had similar effects on cellular viability at 3 and 6 h treatments. Cell viability of Ca(OCl)(2)at concentrations of 0.0125%, 0.025%, 0.05%, or 0.125% was significantly lower than that of NaOCl at 24 h treatment (P < .05).Comparing Ca(OCl)(2)and NaOCl treatments at all time points and concentrations, the damaged cell number of Ca(OCl)(2)was almost fourfold higher than that of NaOCl. In conclusion, both, NaOCl and Ca(OCl)(2)solutions were cytotoxic and genotoxic to NIH3T3, however, Ca(OCl)(2)had a significantly higher damaged cell percentage than NaOCl at all time points and concentrations investigated.
机译:抗微生物灌溉溶液在临床环境下广泛使用。它们对牙科组织的影响是最近研究的主题,旨在为临床使用的更安全的灌溉剂。在这方面,我们的目标是评估细胞毒性和钙氯酸盐(Ca(OCL)(2))溶液的细胞毒性和遗传毒性以及小鼠胚胎成纤维细胞(NiH3T3)的脱钙溶液。首先,用NaOCl或Ca(2)以3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四唑鎓溴(MTT )测定,然后根据细胞增殖图计算细胞活力。其次,通过彗星测定评估基因毒性。数据通过Tukey的测试进行了统计分析(P <.05)。 NaoCl和Ca(OCL)(2)对3和6小时治疗的细胞活力具有类似的影响。 Ca(OCL)(2)的细胞活力在0.0125%,0.025%,0.05%或0.125%的浓度下显着低于24小时治疗的NaoCl(P <.05).paring Ca(OCL)(2 )在所有时间点和浓度下,NaoCl治疗,Ca(OCL)(2)的受损细胞数几乎高于高于NaoCl的细胞数。总之,NaOCl和Ca(OCL)(2)溶液均为细胞毒性和基因毒性,但是,在所有时间点的植物中具有比NAOCL显着更高的损伤细胞百分比。

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