In vitro differentiation and maturation of mouse embryonic stem cells into hepatocytes

摘要

The present invention provides a method for preparing a mature hepatocyte from an embryonic stem cell in vitro, comprising: (a) culturing the embryonic stem cell so as to differentiate into an endodermal cell; (b) isolating the endodermal cell from a population of the differenciated cell; and (c) culturing the isolated endodermal cell in the presence of a Thy 1-positive mesenchymal cell.