Analysis of clinical important of LncRNA-HOTAIR gene variations and ovarian cancer susceptibility

摘要

LncRNAs are of functional long non-coding RNAs, which have been shown to be involved in critical pathways in cancer development.LncRNA-HOTAIRgene overexpression has been reported in several cancers. The aim of this study was to evaluate the associations between two variants oflncRNA-HOTAIR(rs1899663 G>T and rs4759314 A>G) gene polymorphisms and the risk of ovarian cancer (OC) susceptibility. We performed a case and control analysis on two hundred individuals consisting of 100 cases with OC and 100 women cancer-free in East Azerbaijan of Iranian population. To evaluate the association between two SNPs oflncRNA-HOTAIRwith the risk of OC susceptibility used the polymerase chain reaction-restriction fragment-length polymorphism (PCR-RFLP) method. We revealed that two SNPs in thelncRNA-HOTAIRgene were significantly associated with the risk of OC. The dominant model of rs4759314 inlncRNA-HOTAIR(AA vs. GA/GG) showed a significantly increased risk with an OR of 10.036 (CI 2.253-44.712, P = 0.000); the recessive model of rs1899663 (TT vs. GT/GG) revealed a significantly increased risk with OR of 0.910 (CI 0.856-0.968; P = 0.002). In addition, our findings demonstrated that the 4759314G (OR 13.500; CI 3.146-57.940; P = 0.000) and 1899663T (OR 3.273; CI 1.433-7.475; P = 0.003) alleles are increased the risk of OC susceptibility. Our findings provide evidence that the specific genetic variants inlncRNA-HOTAIRgene may affect OC susceptibility in an Iranian population.