Comparison between mammalian cell and bacterial cell-free system for high-throughput expression of linear PCR-amplified immunoglobulin genes

摘要

BACKGROUND: Monoclonal antibodies have become the most crucial and fastest growing group of protein therapeutics dedicated by modern biotechnology. These molecules represent a powerful reagent not only as a weapon to fight against lethal pathogens, but also as tools for many molecular immunology investigations. Therefore, the development of a high-throughput procedure to generate the antibodies in quite an amount is highly required. Two rapid methods, mammalian cells-based expression and bacterial cell-free transcription/translation system, have been developed for the rapid generation of a functional monoclonal antibody. However, the use of both methods to express the same linear polymerase chain reaction (PCR)-amplified immunoglobulin genes (Ig-genes) for generation of a functional monoclonal antibody fragment (Fab) has not been compared yet.