An in vitro focus on doxorubicin hydrochloride delivery of novel pH-responsive poly(2-succinyloxyethylmethacrylate) and poly[(N-4-vinylbenzyl),N,N-diethylamine] diblock copolymers

摘要

Novel pH-responsive poly(2-succinyloxyethylmethacrylate)-b-poly[(N-4-vinylbenzyl),N,N-diethylamine] [poly(SEMA-b-VEA)] diblock copolymers were synthesized via reversible addition fragmentation transfer (RAFT) polymerization to investigate their self-assembly micellar behavior. The self-assembly behaviors of synthesized diblock copolymers with distinct molecular weights (labeled (1) to) were confirmed by H-1 NMR spectroscopy, TEM and dynamic light scattering measurements. Doxorubicin hydrochloride (DOX) loading capacity was evaluated, and the in vitro cytotoxicity effect of DOX-loaded diblock copolymer was also studied by assessing the survival rate of the breast cancer cell line MCF-7 with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The results exhibited remarkable controlled release in the MTT assay. The DOX encapsulation efficiency was calculated to be 96.4%. The size and zeta potential of DOX-loaded poly(SEMA-b-VEA) diblock copolymers were 204nm and +5.7mV at a pH of 7.4. DOX release values after 440h at pH7.4, 5.4 and 4 were 22.15%, 31.43% and 47.06%, respectively. The released values of DOX-loaded poly(SEMA-b-VEA) and at pH7.4 were 22.15%, 20.5% and 17.5%, respectively. Cell survival ratios were 18.9%, 23.16% and 16.92% after 72h. Poly(SEMA-b-VEA) copolymers can be considered in nanomedicine applications due to their excellent pH-responsive micellar behavior. (c) 2017 Society of Chemical Industry