An        <fi >in vitro</fi>in vitro        focus on doxorubicin hydrochloride delivery of novel pH‐responsive poly(2‐succinyloxyethylmethacrylate) and poly[(        <fi >N</fi>N        ‐4‐vinylbenzyl),        <fi >N</fi>N        ,        <fi >N</fi>N        ‐diethylamine] diblock copolymers

Ghamkhari Aliyeh; Massoumi Bakhshali; Agbolaghi Samira

摘要

Abstract > Novel pH‐responsive poly(2‐succinyloxyethylmethacrylate)‐ <fi>b</fi> ‐poly[( <fi>N</fi> ‐4‐vinylbenzyl), <fi>N</fi> , <fi>N</fi> ‐diethylamine] [poly(SEMA‐ <fi>b</fi> ‐VEA)] diblock copolymers were synthesized via reversible addition fragmentation transfer (RAFT) polymerization to investigate their self‐assembly micellar behavior. The self‐assembly behaviors of synthesized diblock copolymers with distinct molecular weights (labeled (1) to) were confirmed by 1 H NMR spectroscopy, TEM and dynamic light scattering measurements. Doxorubicin hydrochloride (DOX) loading capacity was evaluated, and the <fi>in vitro</fi> cytotoxicity effect of DOX‐loaded diblock copolymer was also studied by assessing the survival rate of the breast cancer cell line MCF‐7 with 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide (MTT) assay. The results exhibited remarkable controlled release in the MTT assay. The DOX encapsulation efficiency was calculated to be 96.4%. The size and zeta potential of DOX‐loaded poly(SEMA‐ <fi>b</fi> ‐VEA) diblock copolymers were 204?nm and +5.7?mV at a pH of 7.4. DOX release values after 440?h at pH?7.4, 5.4 and 4 were 22.15%, 31.43% and 47.06%, respectively. The released values of DOX‐loaded poly(SEMA‐ <fi>b</fi> ‐VEA) and at pH?7.4 were 22.15%, 20.5% and 17.5%, respectively. Cell survival ratios were 18.9%, 23.16% and 16.92% after 72?h. Poly(SEMA‐ <fi>b</fi> ‐VEA) copolymers can be considered in nanomedicine applications due to their excellent pH‐responsive micellar behavior. ? 2017 Society of Chemical Industry </abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> <div class="translation abstracttxt"> <span class="zhankaihshouqi fivelineshidden" id="abstract"> <span>机译：</span><Abstract Type =“Main”XML：Lang =“en”> <标题类型=“main”>抽象</ title> > 新型pH-响应聚（2-琥珀酰氧基乙基甲基丙烯酸甲酯） - <fi> b </ fi> -poly [（ <fi> n </ fi> -4-乙烯基苄基）， <fi> n </ fi> 那 <fi> n </ fi> - 二乙胺] [聚（SEMA- <fi> b </ fi> -VEA）]通过可逆添加碎片转移（筏）聚合来合成二嵌段共聚物，以研究其自组装胶束行为。通过不同分子量（标记（1）至）的合成二嵌段共聚物的自组装行为 1 </ sup> H NMR光谱，TEM和动态光散射测量。盐酸辛酸（DOX）加载能力评价，以及 <fi>体外</ fi> 还通过评估乳腺癌细胞系MCF-7的存活率与3-（4,5-二甲基噻唑-2-基）-2,5-二苯基四唑溴（MTT）测定来研究Dox加载二嵌段共聚物的细胞毒性作用。。结果表现出MTT测定中的显着控制释放。 DOX封装效率计算为96.4％。 DOX加载多（SEMA-）的大小和Zeta电位（SEMA- <fi> b </ fi> -VEA）二嵌段共聚物在pH为7.4的pH值下为204℃和+5.7μmV。在pH？740℃下440℃的DOX释放值分别为22.15％，31.43％和47.06％。 Dox加载多（SEMA-）的释放值（SEMA- <fi> b </ fi> -VEA）和pH？7.4分别为22.15％，20.5％和17.5％。 72℃后，细胞存活率为18.9％，23.16％和16.92％。 poly（sema- <fi> b </ fi> -VEA）由于其优异的pH-响应胶束行为，可以在纳米胺类应用中考虑共聚物。还2017年化学工业学会 </ p> </摘要> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> </div> <div class="record"> <h2 class="all_title" id="enpatent33" >著录项</h2> <ul> <li> <span class="lefttit">来源</span> <div style="width: 86%;vertical-align: text-top;display: inline-block;"> <a href='/journal-foreign-24639/'>《Fortschritte der Physik》</a> <b style="margin: 0 2px;">|</b><span>2018年第3期</span><b style="margin: 0 2px;">|</b><span>共9页</span> </div> </li> <li> <div class="author"> <span class="lefttit">作者</span> <p id="fAuthorthree" class="threelineshidden zhankaihshouqi"> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Ghamkhari Aliyeh&option=202" target="_blank" rel="nofollow">Ghamkhari Aliyeh;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Massoumi Bakhshali&option=202" target="_blank" rel="nofollow">Massoumi Bakhshali;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Agbolaghi Samira&option=202" target="_blank" rel="nofollow">Agbolaghi Samira;</a> </p> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zkzz" style="display: none;">展开▼</span> </div> </li> <li> <div style="display: flex;"> <span class="lefttit">作者单位</span> <div style="position: relative;margin-left: 3px;max-width: 639px;"> <div class="threelineshidden zhankaihshouqi" id="fOrgthree"> <p>Department of ChemistryPayame Noor UniversityTehran Iran;</p> <p>Department of ChemistryPayame Noor UniversityTehran Iran;</p> <p>Chemical Engineering Department Faculty of EngineeringAzarbaijan Shahid Madani UniversityTabriz Iran;</p> </div> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zhdw" style="display: none;">展开▼</span> </div> </div> </li> <li > <span class="lefttit">收录信息</span> <span style="width: 86%;vertical-align: text-top;display: inline-block;"></span> </li> <li> <span class="lefttit">原文格式</span> <span>PDF</span> </li> <li> <span class="lefttit">正文语种</span> <span>eng</span> </li> <li> <span class="lefttit">中图分类</span> <span><a href="https://www.zhangqiaokeyan.com/clc/158.html" title="物理学">物理学;</a></span> </li> <li class="antistop"> <span class="lefttit">关键词</span> <p style="width: 86%;vertical-align: text-top;"> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=pH‐responsive&option=203" rel="nofollow">pH‐responsive;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=diblock copolymer&option=203" rel="nofollow">diblock copolymer;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=drug delivery&option=203" rel="nofollow">drug delivery;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=MTT assay&option=203" rel="nofollow">MTT assay;</a> </p> <div class="translation"> 机译：pH-响应;二嵌段共聚物;药物递送;MTT测定; </div> </li> </ul> </div> </div> <div class="literature cardcommon" id="literaturereference" style="display:none"> <div class="similarity "> <h3 class="all_title" id="enpatent111">引文网络</h3> <div class="referencetab clearfix"> <ul id="referencedaohang"> <li dataid="referenceul">参考文献</li> <li dataid="citationul">引证文献</li> <li dataid="commonreferenceul">共引文献</li> <li dataid="commoncitationul">同被引文献</li> <li dataid="tworeferenceul">二级参考文献</li> <li dataid="twocitationul">二级引证文献</li> </ul> </div> <div class="reference_details" id="referenceList"> <ul id="referenceul"></ul> <ul id="citationul"></ul> <ul id="commonreferenceul"></ul> <ul id="commoncitationul"></ul> <ul id="tworeferenceul"></ul> <ul id="twocitationul"></ul> </div> </div> </div> <div class="literature cardcommon"> <div class="similarity "> <h3 class="all_title" id="enpatent66">相似文献</h3> <div class="similaritytab clearfix"> <ul> <li class="active" >外文文献</li> <li >中文文献</li> <li >专利</li> </ul> </div> <div class="similarity_details"> 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An in vitroin vitro focus on doxorubicin hydrochloride delivery of novel pH‐responsive poly(2‐succinyloxyethylmethacrylate) and poly[( NN ‐4‐vinylbenzyl), NN , NN ‐diethylamine] diblock copolymers

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